胶原-壳聚糖复合支架用作组织工程心瓣膜的可行性  

Feasibility of collagen-chitosan complex scaffold as tissue engineered heart valve

作　　者：付建华[1,2] 赵曼[3] 杨达宽[4] 黄云超[5] 

机构地区：[1]聊城市人民医院胸心外科 [2]昆明医学院第二附属医院胸心外科,云南省昆明市650101 [3]聊城市人民医院信息科 [4]昆明医学院第二附属医院胸心外科 [5]昆明医学院第三附属医院胸心外科

出　　处：《中国组织工程研究与临床康复》2007年第48期9751-9753,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:构建组织工程心脏瓣膜的先决条件之一是构建最佳解剖结构和性能的支架材料,拟证明胶原-壳聚糖复合支架可以作为组织工程心脏瓣膜的最佳支架材料。方法:实验于2004-03/2005-02在中国科学院昆明动物研究所完成。将胶原与壳聚糖分别按7∶1,5∶1,3∶1质量比混合,冷冻干燥法成膜,碾压平整后制成胶原-壳聚糖复合多孔支架。将复合支架制成直径1.0cm的圆片,依次经过固定、冲洗、脱水、透明、浸蜡、包埋、切片、展片与烘片行一般物理性状观察、苏木精-伊红染色和Masson染色观察内部结构;再将另一圆片依次经过固定、洗涤、脱水、置换、浸透、包埋后聚合、临界点干燥、喷镀、安置处理后制成超薄切片进行电镜扫描,观察其超微结构特性。结果:胶原-壳聚糖复合支架外观呈微黄色,半透明状,可见微孔状结构,柔韧适度,富有弹性,抗张强度约为4.0MPa,孔径大小基本一致,保持在80～260μm,孔隙率在95%以上,且孔与孔相互贯通,呈立体网状结构。结论:胶原-壳聚糖复合支架的结构和物理性状符合组织工程心瓣膜材料的要求。AIM： Constructing tissue engineered heart valve is based on a precondition of an optimal scaffold with anatomic structure and performance. This study explores feasibility of collagen-chitosan complex scaffold as tissue engineered heart valve. METHODS： The experiment was carried out in the Kunming Animal Institute, Chinese Academy of Sciences from March 2004 to February 2005. Collagen and chitosan were mixed according to proportions to 7：1, 5：1, 3：1, and collagen-chitosan complex porous scaffold was established after drying freeze and grinding fiat. Then scaffolds were prepared into a disc of diameter 1.0 cm, followed by in turn fixing, flushing, dehydrating, being transparent, wax impregnating, embedding, slicing, spreading and toasting for general physical observation, hematoxylin-eosin staining and Masson staining, to observe the constructive feature; again, another disc was fixed, washed, dehydrated, replaced, soaked, aggregated after embedding, dried on critical point, sprayed plating and prepared into ultra-thin section for the detection of ultra-structure feature under scanning electronic microscope. RESULTS： Collagen-chitosan complex scaffold was slightly yellow on surface, semi-transparent, micro-holy, flexible moderately and elastic. Anti-tension was about 4.0 MPa, hole size was fundamentally consistent, keeping range from 80 to 260 μm, porosity exceeded 95%, holes were interpenetrate and appeared spatial reticular structure. CONCLUSION： With the structure and physics characteristics, collagen-chitosan complex scaffold is suitable for tissue engineered heart valve materials.

关 键 词：胶原-壳聚糖复合支架 组织工程 心脏瓣膜 

分 类 号：R318.08[医药卫生—生物医学工程]