组织工程支架材料三维多孔聚乙烯醇水凝胶的制备  

Preparation of polyvinyl alcohol hydrogel with three-dimension porous structure as scaffold material for tissue engineering

作　　者：段志军[1] 张振方[1] 童昕[1] 

机构地区：[1]沈阳理工大学材料学院中加人工心脏研究中心,辽宁省沈阳市110000

出　　处：《中国组织工程研究与临床康复》2007年第48期9762-9764,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:制备组织工程支架材料,使其内部具有三维多孔结构,以种植和培养细胞。方法:利用氯化钠在二甲基亚砜/水溶液中的溶解度很小的特点,在聚乙烯醇的二甲基亚砜/水溶液中加入不同量的氯化钠颗粒,搅拌均匀而后迅速冷冻,待聚乙烯醇的二甲基亚砜/水凝胶形成后,将凝胶置于蒸馏水中1周,从而将该凝胶中的二甲基亚砜置换出并且溶出氯化钠,由此得到具有三维多孔结构的聚乙烯醇水凝胶。结果:①聚乙烯醇水凝胶的力学性能包括断裂强度,断裂伸长率,弹性模量等和制孔前相比基本没有变化,仍然符合作为组织工程支架材料的要求。②干燥速率随着孔隙率的增大先是降低后又上升。③水凝胶内部可以清晰地观察到三维孔隙结构,孔隙的大小符合作为组织工程支架材料的要求。结论:实验经过改进工艺方法,得到了具有三维多孔性的聚乙烯醇复合水凝胶。AIM：To prepare the tissue-engineered scaffold material with three-dimension （3D） porous structure to seed and culture cells. METHODS： Due to the small solubility of sodium chloride in dimethyl sulfoxide/water solution, different quantities of sodium chloride particles were added into the dimethyl sulfoxide/water solution dissolving polyvinyl alcohol （PVA）. They were mixed and frozen immediately. When the PVA hydrogel was formed, it was put into distilled water for one week to remove the dimethyl sulfoxide and sodium chloride particles. The PVA hydrogel with 3D porous structure was obtained. RESULTS： （1）PVA hydrogel remained the mechanical performance including breaking strength, extensibility at breaking and Young＇s modulus as the one had no holes. It was still accorded with the requests of tissue engineering. （2）The dry rate was decreased and then increased as the porosity increase. （3）3D porous structure was observed clearly in the internal of PVA hydrogel, and the size of holes was accorded to the request of materials for tissue engineenng. CONCLUSION： PVA hydrogel with 3D porous structure is successfully prepared by using the modified technique.

关 键 词：支架材料 PVA水凝胶 三维多孔结构 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]