氦氖激光照射对兔正畸牙周组织血管改建的影响(英文)  

Effects of He-Ne laser irradiation on the periodontal blood vessel remodeling during orthodontic movement in rabbits

作　　者：孙新华[1] 王敏[1] 王旭[1] 

机构地区：[1]吉林大学口腔医学院正畸科,吉林省长春市130021

出　　处：《中国组织工程研究与临床康复》2007年第49期10009-10013,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：吉林大学创新基金(02129)~~

摘　　要：背景: 血管改建是正畸牙周组织改建的基础, He-Ne 激光照射能否促进正畸牙周组织的血管改建, 从而加速正畸牙周组织改建的进程呢?目的: 观察 He-Ne 激光照射对兔正畸牙周组织血管改建的影响, 探讨其可能的作用机制。设计: 随机对照观察。单位: 吉林大学实验动物中心。材料: 选用 35 只大耳白兔, 体质量 2.0 kg(由吉林大学实验动物中心提供 , 许可证号为 SYXK ( 吉 ) 2002-0002, 生产许可证号码为SCXK2003-0003)。实验过程中对动物的处置符合动物伦理学标准。上海产 HNGSQ-1 型 He-Ne 激光治疗仪, HPIAS-1000 图像分析系统为同济医科大学生产。CD34 单克隆抗体, 产品编号为 MAB-0034,由福州迈新公司提供。血管内皮细胞生长因子单抗, 产品编号为 MS-350, 由福州迈新公司提供。方法: 实验于 2003- 05/2003- 12 于吉林大学实验动物中心省级实验室完成。①采用随机数字表法将 35 只大耳白兔随机分成 7 组: 未加力组、加力 1, 3, 5, 7, 14 及 21 d 组, 每组 5 只。加力各组动物于双侧上颌第一磨牙至切牙间拴结不锈钢螺簧, 0.08 N 力拉上颌第一磨牙向近中移动,各组动物采用自身对照, 右侧为氦氖激光照射侧, 左侧为对照侧。用波长632.8 nm, 激光功率 20 mW, 能量密度 2.50 J/cm2 的氦氖激光照射加力各组兔右侧颊部。未加力组无干预措施, 饲养 1 周后取材, 加力各组在加力后各时间点取材。②各组均制作以上颌第一磨牙为中心的、近远中方向的组织切片, 行 CD34 及 VEGF 免疫组化染色, 检测各组牙周组织压力区与张力区微血管密度与微血管面积, 计算机图像分析系统测量灰度值评价 VEGF 的染色强度。主要观察指标: ①血管内皮生长因子免疫组化染色的灰度积分。②微血管密度及微血管面积。结果: 实验兔 35 只均进入结果分析。①微血管密度与微血管面积检测结果: 加力 3, 5, 7, 14 及 21 d 组 He-BACKGROUND： Blood vessel remodeling is the basis of the remodeling of orthodontic periodontal tissues. Can He-Ne laser irradiation promote blood vessel remodeling and then accelerate the process of the remodeling of orthodontic periodontal tissues or not？ OBJECTIVE： To investigate the effects and action mechanisms of He-Ne laser irradiation on the blood vessel remodeling of orthodontic periodontal tissues of rabbits. DESIGN： Randomized controlled study.SETTING ： Animal experimental center of Jilin University.MATERIALS： Thirty-five flap-eared rabbits weighting 2.0 kg were provided by Animal Experimental Center of Jilin University, license No. SYXK（Ji）2002-0002, production license No. SCXK2003-0003. Animal intervention met animal ethical standard. HNGSQ-1 type of He-Ne laser therapeutic instrument was produced in Shanghai. HPIAS-1000 image analytical system was produced by Tongji Medical University. CD34 monoclonal antibody with the product number of MAB-0034 and vascular endothelial growth factor monoclonal antibody with the product number of MS-350 were offered by Fuzhou Maixin Company. METHODS： Experiments were performed at the Provincial Laboratory of Animal Experimental Center of Jilin University from May to December 2003. （1）Thirty-five flap-eared rabbits were randomly divided into seven groups, an unforced group and forced 1-, 3-, 5-, 7-, 14- and 21-day groups with 5 rabbits in each group. A stainless steel closing coil spring was deposited between the maxillary first molar and the corresponding maxillary incisor bilaterally, delivering 0.08 N force. The right maxillary of rabbits of each forced group was irradiated by He-Ne laser, and the left side was as control. The cheek corresponding to the right maxillary first molar was selected as irradiation spot and then received He-Ne laser local irradiation. He-Ne laser＇s wavelength is 632.8 nm, power output of 20.0 mW and energy density of 2.50 J/cm^2. Animals of the unforced group did not proceed with anything, sacrificed after raising f

关 键 词：HE-NE激光 口腔正畸 血管改建 CD34 血管内皮生长因子 免疫组化 

分 类 号：R681.531[医药卫生—骨科学]