磷酸二酯酶5选择性抑制剂zaprinast对小鼠卵母细胞成熟的影响  

Effects of Phosphodiesterase Type-5 Inhibitor on Mouse Oocyte Maturation In Vitro

作　　者：卜淑敏[1] 张颢[1] 季刚[1] 

机构地区：[1]首都体育学院科研处重点实验室,北京100088

出　　处：《中国比较医学杂志》2007年第12期702-705,共4页Chinese Journal of Comparative Medicine

基　　金：北京市教委科技发展规划资助项目(编号:KM200710029001)

摘　　要：目的研究磷酸二酯酶5(PDE5)选择性抑制剂扎普司托(zaprinast)对小鼠卵母细胞体外成熟的影响。方法采用小鼠卵母细胞自发成熟、促卵泡刺激素(FSH)诱导次黄嘌呤(HX)阻滞成熟以及卵泡卵母细胞成熟三种体外培养模型。结果10μmol/L的zaprinast不仅能显著抑制小鼠卵丘卵母细胞复合体(COCs)和裸卵(DOs)的自发成熟(P<0.01),而且还能显著抑制FSH对HX阻滞的COCs的以及卵泡卵母细胞成熟的促进效应(P<0.01)。结论PDE5能促进小鼠卵母细胞的体外成熟。Objective To study the effects of the selective inhibitor of PDE5 on mouse oocyte maturation in vitro. Methods Mouse cumulus-oocyte complexes （COCs） or denuded oocytes （DOs） were collected and cultured in the medium supplemented with the selective inhibitor of PDE5 and the rates of germinal vesical breakdown （GVBD） and first polar body （PB1） extrusion were observed at the end of the culture. In addition, a mouse follicle culture model was also used. Results In isolated oocytes, PDE5 inhibitor zaprinast prevented significantly both spontaneous oocyte meiotic maturation and FSH-induced HX-arrested meiotic maturation. In addition, treated the follicles with the type 5 inhibitor significantly inhibited the follicle-enclosed oocyte maturation induced by FSH. Conclusion Collectively, these results suggest that PDE5 induces mouse oocyte maturation.

关 键 词：磷酸二酯酶 cAMP CGMP 卵母细胞 小鼠 

分 类 号：Q492.5[生物学—生理学]