大鼠松果体N-乙酰基转移酶基因的内源性昼夜表达规律  

Endogenous Circadian Expression of the Arylalkylamine N-acetyltransferase Gene in the Pineal Gland of Rat

作　　者：王国卿[1] 傅春玲[2] 李建祥[2] 杜玉珍[2] 童建[2] 

机构地区：[1]苏州大学医学院基础医学系生理学教研室,苏州215123 [2]苏州大学放射医学与公共卫生学院卫生毒理学教研室,苏州215123

出　　处：《实验动物与比较医学》2007年第4期215-218,共4页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金(30170295);苏州大学医学发展基金(EE134031);青年教师研究基金(Q3134044)项目资助

摘　　要：目的探讨大鼠松果体N-乙酰基转移酶(NAT)基因的内源性昼夜节律表达。方法SD大鼠在持续黑暗(DD)光制下饲养8周后,在一昼夜内每隔4 h采集一组松果体组织,提取总RNA,进行竞争性定量RT-PCR,测定不同昼夜时点(CT)样品中NAT基因mRNA的相对表达量,用余弦函数软件获取节律参数,并经振幅F检验分析是否存在昼夜节律。结果松果体NAT基因mRNA的表达呈现明显的内源性节律振荡(P<0.05),峰值(mRNA水平为1.42±0.11)和谷值(mRNA水平为0.56±0.18)分别位于CT17和CT5,峰值相位-260.85±9.45,振幅0.44±0.09,中值0.99±0.12。结论大鼠松果体NAT基因存在明显的内源性昼夜节律表达,其转录水平在主观黑夜高于主观白天。Objective To investigate the endogenous circadian expression of the Arylalkylamine Nacetyltransferase （NAT） gene in the rat pineal gland. Methods 36 SD rats under constant darkness （DD） for eight weeks were sampled for the pineal gland once every 4 hours in a circadian day. The total RNA was extracted from the samples and the semiquantitative reverse transcription polymerase chain reaction （RT-PCR） was used to determine the temporal changes in mRNA level of the NAT gene during different circadian times （CT）. The data parameters were obtained and analysed by both the cosine function software and the amplitude F test to reveal the circadian rhythm. Results The NAT mRNA level in the pineal gland showed a significantly circadian oscillation （P 〈 0.05）, with the peak （level is 1.42±0.11） at CT17 and trough （level is 0.56±0.18） at CT5. The parameter values of the peak phase, amplitude and mesor were -260.85±9.45, 0.44±0.09 and 0.99±0.12, respectively. Conclusion The endogenous circadian expression of NAT gene in the rat pineal gland is weak during the subjective daytime and robust at subjective nighttime under DD condition.

关 键 词：N-乙酰基转移酶基因 内源性昼夜节律 松果体 大鼠 

分 类 号：R33[医药卫生—人体生理学]