羊种布鲁氏菌疫苗株M5和强毒株16M的比较蛋白质组学研究  

A Comparative study on proteome of Brucella melitensis vaccine strain M5 and a virulent strain,16M

作　　者：赵忠鹏[1] 吴朔[2] 罗德炎[1] 王希良[1] 祝庆余[1] 

机构地区：[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]西北农林科技大学动物科技学院,陕西杨陵712100

出　　处：《中国人兽共患病学报》2007年第12期1172-1175,共4页Chinese Journal of Zoonoses

摘　　要：目的阐明羊种布鲁氏菌疫苗株M5致弱的分子基础,更加深入地理解布鲁氏菌的毒力机制。方法利用双向电泳技术对相同条件下培养的羊种布鲁氏菌疫苗株M5和强毒株16M总蛋白进行分离,两株间差异蛋白点利用基质辅助激光解吸/电离串联飞行时间质谱技术进行鉴定。每个蛋白质点的肽指纹图谱均使用Mascot在NCBInr蛋白质数据库中进行检索。结果共成功鉴定了13个差异蛋白,代表了8种不同的开放阅读框(ORFs),功能涉及能量代谢,应激,物质运输等。结论上述发现为羊种布鲁氏菌疫苗株M5致弱分子基础的阐明提供了新依据。Brucella melitensis is a facultative intracellular bacterial pathogen causing causes brucellosis, a zoonotic disease primarily infecting sheep and goats, and characterized by undulant fever, arthritic pain and neurological disorders in humans. To understand the mechanism of virulence in B. melitensis, two dimension electrophoresis was used to separate the total proteins of a vaccine strain M5 and a virulent strain 16M, which were grown under identical laboratory conditions. The proteome of vaccine strain M5 was computer-assisted analyzed and compared to that of virulent strain 16M. By means of matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI-TOF-MS）, 13 protein spots were identified. Peptide mass fingerprints were searched against the NCBInr database using the Program Mascot from Matrix Science. These proteins represent 8 discrete open reading frames （ORFs）. It was found that certain metabolic pathways may be altered in MS. involving alterations in expressions of 3-hydroxybutyrate dehydrogenase, 4-hydroxybutyrate dehydrogenase, glycerol trinitrate reductase, 3-methyl-2-oxobutanoate dehydrogenase, sugar-binding protein, nickel-binding periplasmic protein precursor and modification of DNA protection during starvation protein, grpE protein was altered in MS. These findings provide new insight for understanding M5 molecular attenuation mechanism.

关 键 词：布鲁氏菌 蛋白质组 双向电泳 质谱 

分 类 号：R378.5[医药卫生—病原生物学]