抗Asia1型口蹄疫病毒单克隆抗体的制备及抗原捕获ELISA检测口蹄疫病毒的研究  

Development of an antigen capture ELISA using monoclonal antibody against foot and mouth disease virus

作　　者：周国辉[1] 李万[1] 赵磊[1] 于力[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/大动物传染病研究室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2007年第12期960-964,共5页Chinese Journal of Preventive Veterinary Medicine

摘　　要：本研究用纯化的Asial型口蹄疫病毒（Footandmouthdiseasevirus，FMDV）免疫BALB／c小鼠，按常规单克隆抗体技术方法，经筛选获得6株能稳定分泌抗Asial型FMDV单抗的杂交瘤细胞株。以牛抗口蹄疫病毒IgG为捕获抗体，选择一株单抗（184）用辣根过氧化物酶标记（HRP-184）作为检测抗体，建立了检测Asial型FMDV的抗原捕获ELISA方法。该方法可检出0．5859μg纯化Asial型FMDV抗原和2．5×10^3TCID50病毒，对O型FMDV、牛结核病、牛肺疫、牛流热、赤羽病、牛传染性鼻炎等病毒进行检测，均为阴性，无交叉反应发生。本研究建立的FMDV抗原捕获ELISA方法，具有敏感性高、特异性强和重复性好的特点，可用于Asia1型FMDV的特异性检测。BALB/c mice were immunized with purified serotype Asial FMDV. Six hybridoma cell lines that secreted monoclonal antibody against serotype Asial FMDV were obtained. Selected McAb was labeled by HRP and used as detector antibody to develop antigen capture ELISA for detection of serotype Asial FMDV. The purified positive polyclonal antiserum against serotype Asial FMDV was used as capture antibody. The Elisa could detect 0.5859 p.g purified FMDV antigen and 2.5 × 10^3 TCID50 FMDV and showed high specificity with no cross reactions with other viruses such as serotype O FMDV, BEFV, IBRV.

关 键 词：Asia1型 口蹄疫病毒 单克隆抗体 抗原捕获ELISA 

分 类 号：S852.43[农业科学—基础兽医学] S854.44[农业科学—兽医学]