德宏水牛微卫星标记分析的群体遗传变异  

Unfolding of Population Structure in Dehong Buffalo Using Microsatellite DNA Markers

作　　者：杨泽宇[1] 苗永旺[1] 李大林 霍金龙[1] 陈涛[1] 何朝阳 创向辉 汤守锟 

机构地区：[1]云南农业大学动物科学学院,云南昆明650201 [2]云南省家畜改良工作站,云南昆明650021 [3]云南省德宏州畜牧局,云南潞西678400 [4]云南省德宏州动物疾病控制中心,云南潞西678400 [5]云南省德宏州潞西市畜牧站,云南潞西678400

出　　处：《Zoological Research》2007年第6期659-663,共5页动物学研究（英文）

基　　金：国家自然科学基金项目(30660024);云南省应用基础研究计划面上项目(2006C0034M);云南省应用基础研究重点项目(2007C0003Z)

摘　　要：德宏水牛是云南省地方水牛的优良品种之一,为了进一步阐明其群体遗传变异和遗传结构,筛选了分别位于水牛14条染色体上的15对微卫星引物,对德宏水牛81个个体进行了检测分析。共检测到62个等位基因,每个座位等位基因数目从2到6个不等,平均等位基因数为4.13,该水牛群体期望杂合度和多态信息含量分别为0.6520±0.1526和0.5863±0.1789,各座位的遗传分化系数在0～0.0919之间,平均值为0.0202。每个座位的基因流较大,平均12.1502。研究结果表明德宏水牛群体遗传多样性较丰富,亚群间的遗传分化程度低,基因流较大,且很少发生近交。Dehong buffalo are an indigenous buffalo breed in Yunnan province, China. A total of 81 unrelated samples from Dehong buffalo breeds were genotyped using 15 microsatellite DNA markers from 14 chromosomes to estimate genetic diversity, genetic differentiation and gene flow within populations. A total of 62 alleles were detected across the 15 loci assayed. All the loci were polymorphic and the number of alleles ranged from two to six, giving a mean number of 4.13 alleles per locus. The expected heterozygosity and polymorphism information content of 15 microsatellite loci were 0.6520 ± 0.1526 and 0.5863± 0.1789, respectively. Coefficients of genetic differentiation ranged from 0 to 0.0919, with the average of 0.0202. Gene flow at each locus was large, with a mean Nm of 12.1502. The results indicated that the genetic diversity was rich among Dehong buffalo and the genetic differentiation in its sub-populations was relatively low, with larger gene flow and less inbreeding.

关 键 词：德宏水牛 微卫星标记 遗传变异 遗传分化 基因流 

分 类 号：Q953[生物学—动物学] S823[农业科学—畜牧学]