氡暴露后大鼠体内^210Po的含量及分布测定  

Concentration and distribution of ^210 Po in rats exposed to radon

作　　者：潘鹏[1] 杨占山[1] 王天昶[1] 童建[1] 周建伟[2] 

机构地区：[1]苏州大学放射医学与公共卫生学院,215123 [2]南京医科大学公共卫生学院

出　　处：《中华放射医学与防护杂志》2007年第6期534-536,581,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30371226）;国防基础科研资助项目（A3820060138）;江苏省重点实验室开放课题基金资助项目（KJS0622）

摘　　要：目的研究大鼠在实验条件下经氡暴露后体内^210Po的含量及分布。方法15只雄性Wistar大鼠随机分为对照组、小剂量暴露组和大剂量暴露组。暴露组大鼠整体暴露于HD-3型多功能生态氡室，累积暴露量分别为100和200WLM。暴露结束后处死动物，分别取肺、肝及股骨等10种器官和组织，样品经湿式灰化，电化学沉积分离^210Po核素，通过a谱仪测定组织中^210Po含量，并计算组织含量。结果SNK统计分析结果显示，3组之间差异有统计学意义的是股骨、肝、性腺和毛发（P〈0．01）以及脾、肾（P〈0．05）；与对照组相比，比目鱼肌、肺差异具有统计学意义（P〈0．05），但2个暴露组之间差异无统计学意义；其余组织单位组织^210Po含量3组之间差异无统计学意义。大鼠单位组织^210Po含量以脾、毛发和肺最高，与其他器官或组织相比，差异具有统计学意义（P〈0．01）；肝、股骨和性腺单位组织^210Po含量也较高；小肠中含量最低。组织含量则以肝最高，其次是肾、股骨和性腺。结论^210Po主要分布在肺、肝、脾、股骨、性腺以及毛发，各器官或组织单位组织^210Po含量以及组织含量随暴露量增加均相应增加。氡暴露后大鼠体内^210Po的含量及分布测定可为进一步研究氡及其子体致癌效应提供剂量学依据。Objective To study the concentration and distribution of ^210Po in rats exposed to radon and its daughters. Methods Fifteen male wistar rats were randomly divided into three groups, including one control group and two radon exposed groups with the cumulative doses of 100 WLM （low dose） and 200 WLM （high dose）, respectively. Tissue samples containing ^210Po were spontaneously deposited onto silvery discs with the diameter of 20 mm by means of wet ashing and electrodeposition. The concentration of ^210Po in tissues were measured by α spectroscopy, and tissue burden were calculated. Results The concentrations of ^210Po were significantly different among the three dose groups in femur,liver,sex gland and hair （ P 〈 0.01 ） and in spleen and kidney （P 〈 0.05）.The concentrations of ^210Po were different between the exposed groups and the control group in lung and soleus muscle （ P 〈 0.05 ）, but there was no difference between the exposed groups. No significant differences were found in other organs or tissues. The concentrations of ^210Po in lung, spleen and hair were higher than that in liver,bone and sex gland, the lowest was in intestine. The tissue burdens of liver,bone and sex gland were significantly different from those in other organs or tissues. Conclusions ^210Po was mainly distributed in lung,liver, spleen, femur and sex gland. The concentrations of ^210Po in organs or tissues and the tissue burdens were correspondingly increased with the exposure dose of radon and its daughters. The results of this experiment provide a dosimetric basis for further studies on the carcinogenic effect of radon and its daughters.

关 键 词：氡 ^210PO 湿式灰化 电化学沉积 

分 类 号：R686[医药卫生—骨科学]