微波辐射对家兔小脑运动性学习记忆和GluR2蛋白质磷酸化的影响  

Effects of microwave exposure on motor learning and GluR2 phosphorylation in rabbit cerebellum

作　　者：刘勇[1] 王登高[1] 张广斌[1] 周文[1] 杨学森[1] 

机构地区：[1]第三军医大学军事预防医学院劳动卫生学教研室,重庆400038

出　　处：《中华放射医学与防护杂志》2007年第6期599-601,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目（30170237）

摘　　要：目的观察微波辐射对家兔小脑运动性学习记忆功能的损伤效应，检测微波辐射对谷氨酸受体2（GluR2）磷酸化的影响。方法选择建立牢固瞬膜条件反射的家兔，随机分为对照组和微波辐射组（辐射后0、3、24和72h等4个时相点），辐射组给予平均功率密度90mW/cm^2的微波辐射30min，测定辐射后家兔的即刻肛温并计算比吸收率（SAR）值；检测辐射后即刻家兔瞬膜条件反射的变化；蛋白质免疫印迹法检测家兔小脑GluR2及其磷酸化蛋白质的表达变化。结果90mW/cm^2微波辐射后即刻家兔肛温显著升高3．02℃，SAR值为8．74W／kg；瞬膜条件反射在辐射后0h显著降低，3h后基本恢复至正常水平；小脑GluR2的蛋白表达水平无显著变化但GluR2的磷酸化水平在微波辐射后即刻显著降低。结论90mW／cm^2的微波辐射30min能使家兔产生明显热效应，并影响小脑GluR2的磷酸化，最终导致小脑运动性学习记忆功能障碍。Objective To investigate the effects of microwave exposure on motor learning and Glutamate receptor 2（GluR2） phosphorylation in rat cerebellum, ncthods The rabbits were trained for seven days to form eye-blink conditioning,and then divided randomly into control and microwave exposure group （at hours 0,3,24 and 72 subgroups after exposure, respectively）. The rabbits were accepted 90 mW/cm^2 microwave exposure for 30 minutes, and the rectal temperature were detected immediately after exposure and specific absorption rate （SAR） value were calculated, Eye-blink conditioning were detected immediately after exposure, and cerebellar GluR2 protein and GluR2 phosphorylation were detected with Western blotting, Results Rectal temperature of rabbits were increased by 3.02℃ after exposure, and SAR value was 8,74 W/kg, The eye-blink conditioning decreased significantly after exposure, and cerebel]ar GluR2 protein expression had no significant alteration but phosphorylation reduced significantly after exposure, Conclusions 90 mW/cm^2 microwave exposure has injurious effects on cerebellar GluR2 phosphorylation and motor learning.

关 键 词：微波辐射 小脑 运动性学习 谷氨酸受体2(GluR2) 蛋白质磷酸化 

分 类 号：R686[医药卫生—骨科学]