脑脊液14-3-3蛋白定量检测对散发性Creutzfeldt-Jakob病的诊断价值  

Diagnostic value of quantitative test of 14-3-3 protein in the cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease

作　　者：于雪凡[1] 林世和[1] 孙瑞红[1] 

机构地区：[1]吉林大学第一医院神经内科,长春130021

出　　处：《临床神经病学杂志》2007年第6期401-403,共3页Journal of Clinical Neurology

基　　金：国家自然科学基金资助项目(30070266;30470589)

摘　　要：目的探讨脑脊液14-3-3蛋白定量检测对散发性Creutzfeldt-Jakob病(sCJD)的诊断价值。方法采用Capture Assay方法定量检测14例sCJD患者(sCJD组)、10例痴呆患者(OD组)、12例非痴呆患者(ND组)脑脊液14-3-3蛋白的含量。结果脑脊液中14-3-3蛋白含量中位数(MD)sCJD组为40.00ng/mg,OD组为2.65ng/mg,ND组为3.10ng/mg;sCJD组脑脊液中14-3-3蛋白含量明显高于OD组及ND组(均P<0.01)。以脑脊液14-3-3蛋白含量≥9ng/ml为分界值时,其对sCJD诊断的敏感性为86.7%,特异性为86.4%,阳性预测值为81.3%,阴性预测值为90.5%。结论应用Capture Assay方法定量检测脑脊液中14-3-3蛋白含量对于临床可疑sCJD的患者有较高的敏感性和特异性。Objective To explore the diagnostic value of quantitative test of 14-3-3 protein content in cerebrospinal fluid （CSF） in sporadic Creutzfeldt-Jakob disease （sCJD）. Methods The Capture Assay was used to detect the level of CSF 14-3-3 protein in 14 cases of sCJD （sCJD group） , l0 cases of other dementia （OD group） , 12 cases of non dementia （ND group）. Results The media of 14-3-3 protein content was 40. 00 ng/mg in sCJD group, 2.65 ng/mg in OD group, and 3.10 ng/mg in ND group, respectively. It was significantly higher in sCJD group than that in groups OD and ND（ all P 〈0. 01 ）. When the cut-off point of 14-3-3 protein content in CSF was ≥ 9 ng/ml , the sensitivity for diagnosis of sCJD was 86.7% , the specificity was 86.4%. The positive predictive value was 81.3% and the negative predictive value was 90.5%. Conclusion The 14-3-3 protein quantitative test by Capture Assay has a high sensitivity and specificity which supports a clinical diagnosis of sCJD.

关 键 词：CREUTZFELDT-JAKOB病 14-3-3蛋白 

分 类 号：R446.14[医药卫生—诊断学]