六味地黄丸入血成分莫诺苷对大鼠前脂肪细胞增殖与分化的影响  

Effects of the original constituent of Liu-wei Di-huang pill morroniside on the proliferation and differentiation of rat preadipocyte

作　　者：戴冰[1] 肖子曾[1] 刘磊[1] 冷旺[1] 梅君[1] 邹双华[1] 

机构地区：[1]湖南中医药大学

出　　处：《中国临床药理学与治疗学》2007年第11期1245-1249,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖南省教育厅资助项目(05C363)

摘　　要：目的:测定大鼠口服六味地黄丸后血清中莫诺苷浓度,并探讨莫诺苷对大鼠前脂肪细胞增殖与分化的影响。方法:反相高效液相色谱法检测大鼠口服六味地黄丸后血清中莫诺苷浓度;原代培养大鼠前脂肪细胞;以四甲基偶氮唑盐(MTT)方法检测莫诺苷对大鼠前脂肪细胞增殖的影响;以油红O染色方法检测其对大鼠前脂肪细胞分化过程中的细胞内脂肪积聚的影响。结果:莫诺苷与血清中其它成分较好分离。在250～2000ng范围内线性关系良好(r=0.9991),平均回收率为95.27%,莫诺苷在大鼠血中的平均血药浓度为(16.92±3.63)μg/mL;8.5～68.0μg/mL的莫诺苷促进大鼠前脂肪细胞的增殖,抑制其分化过程中的脂肪积聚。结论:莫诺苷可能为六味地黄丸的体内直接作用物质之一;有效成分分离测定与药效学研究相结合的方法将有助于阐明六味地黄丸的有效成分及作用机理。AIM： To study the concentration of morroniside in rat serum after oral administration of Liu-wei Di-huang pills（LDP）, to investigate the effect of morroniside on proliferation and differentiation of rat preadipocytes. METHODS： The determination of morroniside concentration in rat serum was taken on RP-HPLC after taking LDP, rat preadipocytes were primary cultured, its proliferation was detected by MTT method, its differentiation was tested by Oil Red 0 staining. RESULTS： The linear range of morroniside was 250 - 2 000 ng （r = 0.9991）, the average recovery was 95.27 %; morroniside of 8.5 -68.0 μg/mL could stimulate rat preadipocyte proliferation, inhibited its lipid accumulation. CON- CLUSION： Morroniside could be one of the original constituents migrating to blood of LDP; the method combined pharmacodynamics with detemaination and isolation of constituents migrating to blood could be helpful for illumi- nating the active components and mechanism of LDP.

关 键 词：六味地黄丸 莫诺苷 大鼠前脂肪细胞 细胞增殖 细胞分化 

分 类 号：R965.2[医药卫生—药理学]