抗眼镜王蛇毒卵黄抗体的免疫活性及其对眼镜王蛇毒的中和作用  

Immunoreactivity and neutralization against king cobra venom of anti-king cobra venom IgY

作　　者：王桂平[1] 刘新艳[2] 朱柳[2] 余清声[2] 

机构地区：[1]广州医学院护理学院 [2]广州医学院药物研究中心

出　　处：《中国临床药理学与治疗学》2007年第11期1250-1254,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广州科技局应用基础研究基金项目(2002J1C0171)

摘　　要：目的:探讨抗眼镜王蛇毒卵黄抗体的免疫活性及其在体内外对眼镜王蛇毒的中和作用。方法:采用嗜硫色谱法一步分离纯化抗眼镜王蛇毒卵黄抗体,通过间接ELISA法对制备的抗眼镜王蛇毒卵黄抗体进行免疫活性检测,采用小鼠体内外保护实验评估抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒的中和作用。结果:嗜硫色谱法制备的卵黄抗体具有良好的免疫活性,并且对眼镜王蛇毒素显示较好的中和效应,在体外6mg/kg抗眼镜王蛇毒卵黄抗体可有效地中和约4LD50(约1.6mg/kg)的眼镜王蛇毒素,而在体内可有效地中和约3LD50(约1.2mg/kg)的眼镜王蛇毒素。结论:抗眼镜王蛇毒卵黄抗体对眼镜王蛇毒具有良好的中和作用,本项目为抗眼镜王蛇毒卵黄抗体的进一步研究开发提供实验依据。AIM： To explore the immunoreactivity and neutralization against king cobra venom of anti-king cobra venom lgY. METHODS： The anti-king cobra venom IgY was developed by thiophilic adsorption chromatography . The biological activity of anti-king cobra venom lgY was detected by indirect ELISA. In vivo and in vitro protective experiments in mice were used for evaluating the neutralization against king cobra venom of anti-king cobra venom IgY. RESULTS： The anti-king cobra venom lgY developed by thiophilic adsorption chromatography showed good biological activity and immunoreactivity, and it also neutralized king cobra venom well. A dose of 6 mg/kg anti-king cobra venom lgY could neutralize 4 LD50 （about 1.6 mg/kg）of king cobra venom in vitro, and neutralize 3 LDs0（about 1.2 mg/kg）of king cobra venom in vivo. CONCLUSION： The anti-king cobra venom lgY can neutralize king cobra venom, and the present study provides experiment data for further study of antiking cobra venom IgY.

关 键 词：眼镜王蛇毒 卵黄抗体 免疫活性 中和作用 

分 类 号：R965.2[医药卫生—药理学]