功能碳黑修饰的丝网印刷碳糊电极葡萄糖生物传感器的特性与机理  

Performance and Mechanism of Screen-Printed Carbon Paste Electrodes Glucose Biosensors Based on Functional Carbon Black

作　　者：杨笑鹤[1] 杨强[2] 杨昊[1] 王立[2] 陈裕泉[1] 

机构地区：[1]浙江大学生物医学工程系生物传感器国家重点实验室,杭州310027 [2]浙江大学高分子系聚合反应工程国家重点实验室,杭州310027

出　　处：《分析化学》2007年第12期1751-1755,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(N0.30470461);国家863项目延伸(No.2003AA404180)

摘　　要：从碳黑表面引发苯乙烯磺酸钠的原子转移自由基聚合制备了聚（苯乙烯磺酸钠）改性碳黑（CB-g-PSS），并分别以掺杂和沉积两种不同方式修饰电极的生物敏感膜，再在生物敏感膜上吸附固定葡萄糖氧化酶，制作了两种葡萄糖氧化酶传感器，得到了不同的效果。实验结果表明，将CB-g-PSS与成膜材料掺杂制作的生物传感器与无修饰传感器相比，响应灵敏度下降了1／3；将CB-g-PSS沉积修饰丝网印刷碳糊电极制作的传感器与无修饰传感器相比，响应灵敏度提高了2倍，且对1．1～33．3mmoL／L的葡萄糖待测样本，RSD均〈7％，稳定性良好，有较高的应用价值。通过实验分析了CB-g-PSS以不同方式修饰电极的T作机理，结果表明，选择正确的修饰方式，能够发挥CB-g-PSS的导电效应及纳米效应，使其有利于酶的固定，提高响应灵敏度并改善酶促反应动力学特性。Poly （sodium 4-styrenesulfonate ） （PSS） was grafted onto carbon black surface by ambient surface-initiated atom radical transfer polymerization. Biological sensitive film of glucose biosensors was modified respectively by two methods： doping and deposition. Then glucose oxidase was immobilized on it. So two kinds of glucose biosensors were prepared, and different effects were achieved. The experiments indicated that the sensitivity of biosensor doped with CB-g-PSS was 1/3 time of unmodified; but the sensitivity of that deposed with CB-g-PSS was 2 times of unmodified. Measurable linear range is 1.1 mmol/L - 33.3 mmol/L with RSD 〈7%. Through analyzing the mechanism of electrodes modified with CB-g-PSS by different meth- ods, the results revealed that conductivity and nanometer property of CB-g-PSS benefit to immobilizing enzyme, improving kinetic process of enzymatic reaction and increasing the sensitivity of biosensors.

关 键 词：生物传感器 碳黑 纳米粒子 固定化 酶 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]