麦尔多拉蓝作为电子介体的一次性血清酒精生物传感器  

Disposable Blood Alcohol Biosensor Based on Meldola's Blue as Electron Transfer Mediator

作　　者：罗鹏[1] 谢国明[1] 宋方洲[2] 邓世雄[3] 徐华健[4] 

机构地区：[1]重庆医科大学检验系临床检验诊断学省部共建教育部重点实验室 [2]重庆医科大学基础医学院生物化学与分子生物学重庆市重点实验室 [3]重庆医科大学基础医学院法医研究所 [4]重庆医科大学附属第一医院,重庆400016

出　　处：《分析化学》2007年第12期1801-1804,共4页Chinese Journal of Analytical Chemistry

基　　金：重庆医科大学创新基金资助项目(No.CX100532)

摘　　要：基于丝网印刷技术制备一次性碳电极，用Nation固定麦尔多拉蓝（MS）作为电子介体，通过交联法用戊二醛将乙醇脱氢酶和氧化型辅酶Ⅰ固定于Nation-MB修饰的电极上，制备一次性血酒精生物传感器。该传感器表现出良好的特异性、灵敏度和准确性。传感器响应电流与血清酒精浓度在2．0×10^-4～2．5×10^-3mol／L之间呈现良好的线性关系；检出限为1．3×10^-4mol／L，达到95％稳态响应时间不超过20s。探讨了pH、缓冲液、温度及干扰物质等对一次性血酒精生物传感器的影响。将传感器用于血清酒精实际样品的测定，取得了满意的结果。A disposable blood alcohol biosensor based on screen printing technique was developed. This biosensor comprises a screen-printed carbon electrode modified with alcohol dehydrogenase, nicotinamide adenine dinucleotide cofactor Meldola＇s blue adsorbed on Nation film. It presented a good specificity, sensitivity and accuracy. Moreover, the biosensor showed a wide linear response range of 2.0 × 10^-4 -2.5 × 10^-3mol/L. The detection limit was 1.3× 10^-4 mol/L and the response time was less than 20 s. The biosensor response was investigated in terms of pH, buffer solution, temperature and the other interferents. The biosensor was applied for measuring serum alcohol and satisfactory result was obtained.

关 键 词：乙醇脱氢酶 氧化型辅酶Ⅰ 血酒精检测 麦尔多拉蓝 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]