罗汉果皂苷Ⅲ的人肠内细菌生物转化  

Biotransformation of mogroside Ⅲ by human intestinal bacteria

作　　者：杨秀伟[1] 张建业[1] 徐嵬[1] 

机构地区：[1]北京大学药学院天然药物学系

出　　处：《北京大学学报（医学版）》2007年第6期657-662,共6页Journal of Peking University:Health Sciences

基　　金：国家高技术研究发展计划863重点项目基金(2002AA2Z343C);北京市科技专项(Z0004105040311)资助~~

摘　　要：目的:探讨人肠内细菌对中药罗汉果中罗汉果皂苷Ⅲ结构的生物转化,确定转化产物的结构,为原形化合物罗汉果皂苷Ⅲ在肠内吸收的评价提供科学依据。方法:采用人肠内细菌与罗汉果皂苷Ⅲ在厌氧条件、37℃共温孵培养的方法对其结构进行生物转化,通过硅胶柱色谱技术分离、纯化生物转化产物,应用红外光谱(infra-red spectrometry,IR)、核磁共振波谱(nuclear magneticresonance spectrometry,NMR)和质谱(mass spectroscopy,MS)等技术方法确定生物转化产物的结构。结果:罗汉果皂苷Ⅲ可由人肠内细菌依次脱去C-3葡萄糖基和C-24龙胆二糖基转化产生罗汉果皂苷ⅡA1和罗汉果醇。结论:罗汉果皂苷Ⅲ可被人肠内细菌进行生物转化,产生次级苷罗汉果皂苷ⅡA1及其苷元罗汉果醇。Objective： To investigate the biotransformation of mogroside Ⅲ, one of the main chemical constituent in the fruits of Momordica grosvenori Swingle , by the crude enzymes of human intestinal bacteria, and determine the structure of biotransformation products and provide scientific basis for absorption evaluation of primary mogroside Ⅲ in human intestine. Methods： The mogroside Ⅲ was incubated with crude enzymes of human intestinal bacteria under the anaerobic environment and 37 ℃ condition to transform mogroside m. The biotransformation products were isolated and purified by silica gel column chromatography, and structurally determined by infra-red （IR） and nuclear magnetic resonance （NMR） spectrometry and mass spectroscopy （MS） techniques. Results： Mogroside Ⅲ was converted to mogroside Ⅱ Al and mogrol by successive deglycosylation at C-3 of the glucosyl group and C-24 of the gentiobiosyl group. Conclusion： The human intestinal bacteria showed potent ability to transform mogroside Ⅲ to release secondary glycoside mogroside Ⅱ Al and aglycone mogrol.

关 键 词：罗汉果 皂苷类 肠 细菌 生物转化 

分 类 号：R282.711[医药卫生—中药学]