干扰素-γ对精子顶体酶活力的影响及其作用机制初探  

Effect of interferon-γ on the activity of acrosin in human sperm and its mechanism

作　　者：边淑玲[1] 张丽敏[1] 郝晓敏[1] 娄延平[1] 姜晓姝[1] 张玮[1] 金宏波[1] 

机构地区：[1]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2007年第6期558-560,共3页Journal of Harbin Medical University

基　　金：黑龙江省卫生厅课题资助项目(2005-006)

摘　　要：目的探讨干扰素-γ(IFN-γ)对正常人精子顶体酶(acrosin)活力的影响及其作用机制。方法采用BAEE/ADH联合法测定精子顶体酶活力,用高效液相色谱(HPLC)测定一氧化氮(NO),用生化法测定超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)。用定磷法测定Na+-K+-ATPase和Ca2+-ATPase活性。结果IFN-γ可降低精子顶体酶活力(P<0.05),明显降低精子中Na+-K+-ATPase、Ca2+-ATPase和SOD活性(P<0.001);但显著提高精子NOS活性和NO浓度(P<0.001)。结论IFN-γ可降低精子顶体酶活力,其机制可能与精子中Na+-K+-ATPase、Ca2+-AT-Pase、SOD和NOS活性以及NO浓度等因素有一定关系。Objective To investigate the effect of interferon-gamma （IFN-γ） on the activity of acrosin in human sperm and its mechanism. Methods The acrosin activity was detected by BAEE/ADH method. The nitric oxide （NO） concentration was tested by HPLC method and the activity of superoxide dismutase （SOD）, nitric oxide synthase （NOS）, Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase were tested by the biochemical technique. Results IFN-γ, decreased the activity of acrosin （P 〈 0.05） and the activities of SOD, Na^＋ -K^＋ -ATPase, and Ca^2＋- ATPase （P 〈 0.001 ）, but increased the NOS activity and the NO concentration in human sperm. Conclusion IFN-γ has some inhibitory effect on sperm acrosin activity, which could be attribute to their influence on activities of Na^＋ -K^＋ -ATPase, Ca^2＋ -ATPase SOD, NOS, the NO concentration and so on.

关 键 词：干扰素-Γ 精子 精子顶体酶 

分 类 号：R392.11[医药卫生—免疫学] R339.2[医药卫生—基础医学]