脐血浆对离体大鼠心肌缺血/再灌注损伤的保护作用  

Protective effect of plasma from umbilical cord on myocardial ischemia/reperfusion injury in isolated rats

作　　者：王云英 李鸿珠[2] 王雨[3,3] 林岩[2] 郭津[2] 高秀香[2] 裴天仙[2] 施建华 徐长庆[2] 

机构地区：[1]哈尔滨市南岗区妇产医院,黑龙江哈尔滨150080 [2]哈尔滨医科大学病理生理学教研室,黑龙江哈尔滨150081 [3]哈尔滨工业大学医院,黑龙江哈尔滨150006

出　　处：《哈尔滨医科大学学报》2007年第6期563-565,共3页Journal of Harbin Medical University

基　　金：黑龙江省科技厅国际合作课题资助(WC02303)

摘　　要：目的观察脐血浆(UC)对离体大鼠心肌缺血/再灌注损伤的保护作用并初步探讨其可能机制。方法取Wistar大鼠24只,随机分成正常对照组(Control)、缺血/再灌注损伤组(I/R)和脐血浆保护组(UC)。利用Langendorff离体灌流装置,复制大鼠心肌缺血/再灌注损伤模型。观察冠脉流出液中乳酸脱氢酶(LDH)活性、心肌组织匀浆中SOD活性及心肌超微结构等。结果与Control组相比,I/R组LDH活性明显增高,SOD活性降低,心肌超微结构损伤明显;脐血浆(1∶100)则明显减少LDH的漏出,同时使SOD活性升高及心肌超微结构损伤减轻。结论脐血浆能减轻大鼠心肌缺血/再灌注损伤,其机制可能与脐血浆具有抗氧化、清除自由基的作用有关。Objective To observe the effect of human plasma from umbilical cord on myocardial isehemia/ reperfusion injury in rats and to study its possible mechanism. Methods Twenty-four Wistar rats were randomly divided into 3 groups： control group, isehemie reperfusion （I/R） group, and umbilical cord plasma （UC） group. Myocardial isehemia/reperfusion injury was induced using Langendorff perfusion system. The activity of LDH in coronary effluent and SOD in cardiac tissue, and the cardiac ultrastmcture were examined. Results Compared with the control group, the activity of LDH was increased, SOD enzyme activity was decreased, and injury of cardiac ultrastructure was obvious in I/R group; in UC （ 1 ： 100） group, the level of LDH was decreased and SOD enzyme activity was increased, the myocardial ultrastructure injury was improved, compared with I/R group. Conclusion The plasma of human could alleviate the myocardial ischemia/reperfusion injury in rats, and the underlying mechanism may be related to antioxidation and oxygen free radical scavenging of components in umbilical cord plasma.

关 键 词：脐血浆 心肌 大鼠 缺血/再灌注损伤 氧自由基 

分 类 号：R33-2[医药卫生—人体生理学] R363[医药卫生—基础医学]