猪繁殖障碍疫病的三重PCR法鉴别诊断  

Diagnosis of pig reproductive disturbance diseases by multiplex PCR method

作　　者：周思旋[1] 周碧君[1] 岳筠[1] 徐春志[1] 鲍娟[1] 罗阿东[1] 史开志[1] 陈琼乖[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025

出　　处：《山地农业生物学报》2007年第6期499-503,共5页Journal of Mountain Agriculture and Biology

基　　金：贵州省"十一五"农业科技攻关重大项目子课题资助[黔科合NZ字(2005)3002];贵阳市农业局科技计划项目资助(2007-08);贵州省农业厅兽医科技计划项目资助(2007-06)

摘　　要：为建立一种简便、快速检测猪繁殖与呼吸综合征病毒(PRRSV)、猪细小病毒(PPV)和猪伪狂犬病病毒(PRV)3种病原的多重PCR方法,针对PRRSV N基因、PPV VP 2基因及PRV gp50基因分别合成了1对特异性引物,通过PCR均扩增出分子长377bp、445bp和217bp的DNA条带,与预期扩增片段相符。以3对引物同时对PRRSV、PPV和PRV疫苗株进行多重PCR扩增及反应条件的优化,同时扩增出3条特异性条带。利用所建立的多重PCR方法,对贵州省11个养猪场的40份疑似病料进行检测,结果显示,PRRSV、PPV和PRV的阳性检出率分别为62.5%、17.5%和0%。In order to make a differential diagnosis of PRRSV, PPV and PRV, three pairs of primers were synthesized according to N gene for PRRS,VP2 gene for PPV and gp50 gene for PRV. The specific DNA bands with molecular length of 377bp,445bp and 217bp could be amplified by PCR method,which were in accordance with the expected results. Meanwhile,the reaction conditions have been optimized with three sets of primers by using PRRSV, PPV and PRV vaccines. 40 samples were collected from the diseased pigs and aborted fetuses in Guizhou province and detected by multiplex PCR method. The results showed that the positive rates of PRRSV ,PPV and PRV were 62.5% , 17.5% and 0% respectively.

关 键 词：猪蓝耳病 细小病毒病 伪狂犬病 三重PCR 

分 类 号：S858.28[农业科学—临床兽医学] S858.28[农业科学—兽医学]