外源亚精胺对不同盐浓度下黄瓜幼苗可溶性蛋白表达的影响  

Effects of Exogenous Spermdine on Soluble Protein Expression in Cucumber Seedlings under Different Saline Concentrations

作　　者：杜长霞[1] 李娟[1] 郭世荣[1] 樊怀福[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《武汉植物学研究》2007年第6期586-590,共5页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30571263)

摘　　要：采用营养液水培的方法,以黄瓜品种‘津春2号’为试材,研究了叶片喷施外源亚精胺(Spd)对不同浓度NaCl(0、50、75、100 mmo.lL-1)胁迫下幼苗植株可溶性蛋白表达的影响。结果表明,50 mmol.L-1NaCl胁迫下植株叶片和根系中可溶性蛋白含量提高;75 mmol.L-1和100 mmol.L-1NaCl胁迫明显降低了幼苗植株叶片和根系中可溶性蛋白的含量。不同浓度NaCl胁迫下喷施Spd后可溶性蛋白含量在根系中没有明显变化规律,而叶片可溶性蛋白含量提高。SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)对黄瓜幼苗根系可溶性蛋白分析表明,至少有3种分子量约为61.5、50、47 kD蛋白在50 mmo.lL-1和75 mmol.L-1NaCl胁迫下表达量增强,但100 mmo.lL-1NaCl胁迫下变化不明显;50 mmol.L-1和75 mmol.L-1NaCl胁迫下喷施Spd后61.5 kD和47 kD蛋白表达量明显减弱,50 kD蛋白甚至消失,100 mmol.L-1NaCl胁迫下61.5 kD和47 kD蛋白无明显变化,50 kD蛋白反而增强。Effects of exogenous spermdine application on soluble protein expression in seedlings of cv. ＇ Jinchun No. 2＇ cucumber （Cucumis sativus L. ） were conducted under different salinity treatments by hydroponic culture. The results showed that the soluble protein content both leaves and roots significantly increased under 50 mmol·L^-1, but decreased under 75 mmol·L^-1 and 100 mmol·L^-1 salt treatments. Exogenous 1 mmol·L^-1 spermdine spray applied increased the soluble protein content in leaves, but no significant changes in roots. By SDS-PAGE electrophoresis analysis,61.5 kD, 50 kD and 47 kD protein bands were increasing expressed in roots under 50 mmol·L^-1 and 75 mmol·L^-1 salt concentration, but not obviously expressed under 100 mmol·L^-1 salt concentration. However,when 1 mmol·L^-1 spermdine was applied ,61.5 kD and 47 kD protein bands slimly expressed ,interestingly ,50 kD protein band disappeared even under 50 mmol ·L^-1 and 75 mmol ·L^-1 salt treatments, but increasing salt concentration to 100 mmol·L^-1 ,61.5 kD and 47 kD protein bands had not obvious changes, then 50 kD protein band expressed obviously.

关 键 词：黄瓜 盐胁迫 亚精胺 可溶性蛋白 SDS-聚丙烯酰胺凝胶电泳 

分 类 号：Q945[生物学—植物学] S642.2[农业科学—蔬菜学]