图像反卷积复原技术在玉米45S rDNA转录图式研究中的运用  

The Application of the Image Deconvolution Technique to the Study of the Maize 45S rDNA Transcription Pattern

作　　者：温若愚[1] 唐燕城[1] 马璐[1] 李立家[1] 

机构地区：[1]武汉大学生命科学学院植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2007年第6期612-618,共7页Journal of Wuhan Botanical Research

基　　金：长江学者和创新团队发展计划;国家自然科学创新研究群体科学基金资助项目(30521004);国家自然科学基金资助项目(30571031);湖北省自然科学基金资助项目(2005ABA097)

摘　　要：由于成像焦平面外杂光的干扰,宽场荧光显微镜所得的图像往往较模糊,使其不适合用来观察植物细胞核内的精细结构。该实验讨论了反卷积软件中对图像复原结果有较大影响的几个重要参数的设置。经过合适的图像反卷积复原,由宽场荧光显微镜获取的玉米45S rRNA原位杂交信号图像得以清楚显示。对所得杂交图像的分析表明:玉米45S rDNA转录往往先发生在核仁外围,且随着核仁转录活性的提高逐渐向核仁内部扩散,并最终与5S rRNA一起,在核仁内部的空洞结构中形成成熟rRNA。研究结果显示,反卷积复原可有效提高宽场荧光显微镜所得二维图像的分辨率,从而使宽场荧光显微镜在植物细胞核内精细结构研究中发挥更多的作用。Due to the effect of the out-of-focus fluorescence, images gained by a wide-field fluorescence microscope were usually too hazy to be used in observing the fine structure of the plant nuclei. In this study, we discussed several important parameters which heavily affect the results of image deconvolution. After proper image deconvolution restoration, the images of the maize 45S rRNA in situ hybridization signals were clearly displayed. The analysis of the hybridization images showed that the transcription of the maize 45S rDNA gene was initiated in the dense fibrillar components （ DFC ）, then transferred to the nucleolus inner space as the nucleolus transcription activity increased, and finally combined with the 5S rRNA to form the mature rRNA in the nucleolus cavity. The results showed that the application of the image deconvolution restoration technique could effectively improve the resolution of the two-dimensional image gained by a wide-field fluorescence microscope, and thus made the wide-field fluorescence microscopes more useful in observing the fine structure of the plant nuclei.

关 键 词：反卷积 45S rDNA转录 ImageJ软件 

分 类 号：Q-334[生物学]