线粒体DNA序列在半翅目异翅亚目昆虫分子系统学上的应用  

Application of Mitochondrial DNA Sequences in Molecular Systematics of True Bugs (Hemiptera:Heteroptera)

作　　者：刘会[1] 李正西[1] 李淑娟 彩万志[1] 

机构地区：[1]中国农业大学昆虫学系 [2]Department of Entomology,Purdue University,West Lafayette,IN 47907-2089,USA

出　　处：《Entomotaxonomia》2007年第4期265-274,共10页昆虫分类学报（英文）

基　　金：国家自然科学基金项目(30370161);北京市自然科学基金项目(6042014)

摘　　要：随着PCR技术的发展以及大量DNA序列的累积,昆虫分子系统学近年来快速发展。线粒体DNA(mtDNA)序列相对于核内DNA序列进化速率较快,常被用于昆虫的系统发育研究。本文综述了国内外学者利用各种线粒体DNA序列来研究半翅目异翅亚目昆虫系统发育的研究概况。总结发现,COⅠ、COⅡ、12S rDNA、16S rDNA、Cytb、ND1、ND2和ND5等线粒体区段被用于半翅目异翅亚目系统发育的研究,其中以COI、COⅡ、16S rDNA和Cytb应用最广泛,但目前尚缺乏不同分子标记间的联合分析。进一步的研究最好在选定半翅目异翅亚目昆虫的分类阶元(如科间、亚科间、科内属间、种间或种内)后,集中测定线粒体某几个区段的DNA序列,然后进行单一分析和联合分析,并与传统形态学研究结果进行比较,可望全面分析半翅目异翅亚目昆虫的系统发育关系。The development in the PCR technique and the accumulation of large amounts of DNA sequence data have led to the remarkable recent growth of insect molecular systematics. Mitochondrial DNA evolves faster than the nuclear genome, and it has been useful in phylogenetic studies of families, genera, species, and populations. We review phylogenetic research advances in Heteroptera. Various mitochondrial DNA sequences, CO Ⅰ, COⅡ 12S rDNA, 16S rDNA, Cyt b, ND1 ND2 and ND5, have been used in phylogenetic studies of true bugs. In which COⅠ, COⅡ, 16S rDNA and Cyt b are the most popular markers. At present, the shortage for using these molecular markers is lack of uniting analyses between different markers data. The further study had better choose some taxonomic rank of hemiptera-heteropterans and focus on mensuration of two regions or several regions of mitochondrial DNA sequences, then analyze solely and unite analyses. At last, we analyse the limitation of mitochondrial gene sequence of utilization in molecular systematics, and the development prospect of mitochondrial genes.

关 键 词：半翅目 异翅亚目 线粒体DNA 分子系统学 

分 类 号：Q963[生物学—昆虫学] Q961