上海地区实验用兔线粒体DNA-RFLP分析  

RFLP Analysis of Mitochondrial DNA of Rabbits from Shanghai

作　　者：冯洁[1] 谢建云 胡建华 高诚 

机构地区：[1]上海实验动物研究中心

出　　处：《上海交通大学学报（农业科学版）》2007年第6期547-550,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科技发展基金资助项目(044909004)

摘　　要：提取3个品种实验用兔肝组织中的线粒体DNA,并用ApaI等21种限制性内切酶进行消化。分析上海地区实验用兔mtDNA的多态性。酶切产物经琼脂糖凝胶电泳检测,共检出8种不同的限制性单倍型,少数个体出现的差异,可能来源于几个位点发生偶然突变,但不同兔群的限制性无规律性。结果表明上海地区实验用兔群体mtDNA的遗传多样性贫乏,遗传结构单一,提示所研究的兔种可能有共同祖先,或人工饲养繁殖出现血缘杂交现象。To analyse polymorphism of Mitochondrial DNA of three strain rabbits from Shanghai, mtDNA was extracted from rabbit liver,and the productions were digested by Apa I et al restriction enzymes. Restriction endonuclease fragments were detected by gelose-gel electrophoresis. 8 mtDNA haplotypes were generated by all the enzymes. The resuh showed that the genetic constitutions of rabbits from Shanghai were simplex, the genetic polymorphism were scarce.

关 键 词：兔 线粒体DNA 限制性内切酶片段长度多态性 

分 类 号：S865.19[农业科学—野生动物驯养]