烟草赤星病菌拮抗链霉菌的多重筛选及发酵液理化性质  

Screening of Antagonistic Microorganism against Alternaria alternata and the Physical and Chemical Properties of the Fermented Broth

作　　者：高芬[1] 穆凌霄[1] 魏颖颖[1] 赵秀香[1] 吴元华[1] 

机构地区：[1]沈阳农业大学植物保护学院

出　　处：《华北农学报》2007年第6期171-175,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家"863"项目子专题(2001AA247011);国家烟草专卖局科技项目(国烟科[2002]187号);辽宁省科技攻关项目(2001208001)

摘　　要：以烟草赤星病菌为靶标菌,利用活体对峙拮抗、发酵液抑菌活性和离体叶片防效测定相结合的方法,从土壤中分离得到的371株放线菌中,筛选到2株抑菌效果明显、抗菌谱广、效果稳定的拮抗放线菌菌株。拮抗菌发酵液的理化性质测定表明:两菌株发酵液对温度、紫外线、自然光、酸碱的稳定性及耐贮性和菌株遗传特性虽有一定差异,但均较为稳定,其中,菌株182-2显示出了良好的抑菌防病效果和优良的稳定性,有开发应用价值。Bio-control microorganism against Alternaria alternata were selected by assessing the inhibition zone of living body, fermented broth on agar media and control effect in detached leaves. From 371 strains isolated from soil, two strains No. 182-2 and No. 163 were found to have obvious and stable antagonistic effect to Alternaria alternata. The properties of fermented broth of the two strains are different to heat, UV and natural light, pH, storing time and subculture. Strain 182-2 has superior capacity of inhibiting pathogens, controlling disease and enduring bad conditions.

关 键 词：烟草赤星病菌 拮抗菌 筛选 理化特性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]