过敏小鼠模型Th1/Th2漂移和纠正  

Modulation of the Th1/Th2 bias in the anaphylaxis mice model

作　　者：王钦富[1] 王永奇[1] 郭连英[2] 董妞妞[3] 施广霞[2] 

机构地区：[1]大连大学生物工程学院,大连116622 [2]大连医科大学病理生理教研室,大连116023 [3]大连大学

出　　处：《中国免疫学杂志》2007年第7期597-599,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(No30171140)

摘　　要：目的:研究炒紫苏子醇提物对过敏小鼠模型Th1/Th2漂移和纠正作用。方法:设正常对照组、过敏模型组、炒紫苏子醇提取物0.32、0.64和1.28g/kg各剂量组小鼠共5组。利用流式细胞仪技术测定Th1型细胞因子IL-2、IFN-γ、TNF-α和Th2型细胞因子IL-4、IL-5水平。结果:过敏模型小鼠IFN-γ/IL-4为0.87,而正常小鼠IFN-γ/IL-4为3.93,说明过敏小鼠Th1/Th2异常偏向Th2漂移。0.32、0.64和1.28g/kg各剂量组能明显提高IFN-γ水平(P>0.05、P<0.05和P<0.01),降低IL-4水平(P>0.05、P>0.05和P<0.05),其相应的IFN-γ/IL-4分别为1.92、2.85和3.14。结论:炒紫苏子醇提物能够纠正过敏小鼠Th1/Th2异常偏向Th2漂移,恢复正常,其作用呈剂量依赖关系。Objective ：To detect the modulation of the Th1/Th2 bias by the EtOH ext. of roasted perilla seed（RPS） in the anaphylaxis mice model. Methods ：The mice were divided randomly into 5 groups, namely 1.28, 0. 64 and 0. 32 g/kg EtOH ext. of RPS group, anaphylaxis model and normal control. All the mice except for the normal control were sensitized by immunized intraperitoneally on days 0 and 5 with chicken OVA. The cytokine profile including IFN-γ, TNF-α, IL-2, IL-4, IL-5 in serum of all the mice were evaluated by FCM. Results：The IFN-γ/IL-4 ratio was decreased from 3.93 in the anaphylaxis mice model to 0. 87 in the normal control group. The mice in 0. 32, 0. 64 and 1.28 g/kg EtOH ext of RPS group displayed a down-regulation for serum IL-4 and TNF-α levels and showed increased levels of IFN-γ with the correspondent IFN-γ/IL-4 ratio of 1.92, 2. 85 and 3.14. Conclusion： The EtOH ext. of roasted perilla seed can modulate the Th1/Th2 bias in a dose-dependent manner.

关 键 词：紫苏子 IFN-Γ TNF-α IL-4 IL-5 流式细胞仪 

分 类 号：R396.2[医药卫生—基础医学]