北方回族人MBL54位密码子基因多态性及血浆含量相关性  

The correlation between point mutation of MBL ExonI and its plasma concentration in North Huis

作　　者：李萍[1] 贾天军[1] 石万元 韩瑞[1] 罗强[1] 张庶民[3] 

机构地区：[1]河北北方学院,张家口075029 [2]康保县医院,张家口076650 [3]中国药品生物制品检定所血清室,北京100050

出　　处：《中国免疫学杂志》2007年第7期607-609,共3页Chinese Journal of Immunology

基　　金：河北省自然基金资助项目(C2006000875);863博士基金项目(2003AA2Z3509)资助

摘　　要：目的:初步调查北方地区健康回族人群MBL基因ExonI54位密码子点突变的情况,检测其血浆MBL含量,探讨二者的相关性。方法:用PCR-RFLP方法检测MBL基因点突变频率;用MBL Oligomer ELISA试剂盒测定出血浆MBL的浓度。结果:测得健康回族人该基因突变频率为0.15;血浆MBL含量的平均值为3.40mg/L;二者呈负相关(r=-0.67)。结论:健康回族人MBL基因54位密码子点突变频率与其血浆含量呈负相关。Objective：The major aim of this study is to analyze the point mutation at the codon 54th of MBL in healthy North Huis,and to measure the plasma levels of MBL, and to analyze the association between the mutation frequency and plasma MBL concentrations. Methods：PCR-RFLP was used to detect MBL point mutation. MBL plasma concentrations were measured using MBL Oliger ELISA kit. Results：Frequency of point mutation at the codon 54th of MBL in healthy Huis was 0. 15. The plasma MBL concentration was （3.40 ± 2. 55 ） mg/L. There was negative correlation between MBL concentrations and gene mutation frequency in huis （ r = - 0. 67）. Conclusion：The relationship between frequency of mutation at codon 54 of MBL gene and the plasma MBL concentrations in healthy Huis is negative correlation.

关 键 词：MBL PCR—RFLP 回族 基因多态性 含量 相关性 

分 类 号：R392.11[医药卫生—免疫学]