体外构建复合皮移植后对大鼠创面愈合的影响  

The healing effects of transplanting the composite skin to the rat wounds

作　　者：梁志为[1] 谭谦[2] 陈曦[2] 黄泽春[1] 林子豪[3] 

机构地区：[1]郴州市第一人民医院整形美容科,湖南郴州423000 [2]南京大学医学院附属鼓楼医院整形烧伤科 [3]第二军医大学附属长征医院整形外科

出　　处：《中国美容医学》2007年第12期1687-1689,共3页Chinese Journal of Aesthetic Medicine

基　　金：铁道部科技基金资助项目(J2000Z083);江苏省社会发展基金资助项目(BS99049);江苏省卫生厅科技基金项目(H200159)

摘　　要：目的:观察角质形成细胞和成纤维细胞与无细胞异种真皮基质构成的复合皮移植于全层皮肤缺损创面后的效果,寻找一种新的创面覆盖物。方法:54只SD大鼠作背部全层皮肤缺损创面后分为A、B、C三组,分别以A型复合皮(角质形成细胞+成纤维细胞+无细胞异种真皮基质)、B型复合皮(角质形成细胞+无细胞异种真皮基质)和普通敷料进行移植,术后定期观察创面愈合情况并进行创面收缩率的计算,同时切取创面组织进行组织学检测。结果:三组中,A组的创面愈合及外观情况最好;A组创面收缩率明显低于B、C两组(P<0.05);组织学检测提示A组复合皮上皮分化充分,胶原增生有序,表皮-真皮连接结构重建明显,未见明显的急性期免疫排斥反应。结论:角质形成细胞和成纤维细胞与无细胞异种真皮基质构成的复合皮移植后能改善创面愈合质量,是一种较理想的、可探索的皮肤替代物。Objective To observe the effects of the recombinant composite skin graft consisting of epithelium, fibroblasts and acellular xeno-dermis on full thickness skin loss wounds, so as to find a new wound covering material. Methods Fifty-four SD rats with full thickness skin loss on the back were divided into three groups as A, B and C and grafted with the composite skin A （keratinocytes＋ time points after the operation. Simultaneously, wound tissue samples were harvested for histological examination. Results The wound healing conditions in A group was best in the three groups, the contraction rate in A group was obviously lower than that in B group and C group （P〈0.05）. It was indicated by histological examination that there was full differentiation of epithelium, orderly collagen proliferation and obvious reconstruction of the epithelial-dermal conjunction structure in A group. The was no obvious sign of acute immune rejection. Conclusion The wound covering with recombinant composite skin graft of epithelium, fibroblasts and acellular xeno-dermis could improve wound healing quality. The composite skin could be a new skin substitute.

关 键 词：角质形成细胞 无细胞异种真皮基质 组织工程 皮肤 

分 类 号：Q813.1[生物学—生物工程]