Construction of an electronic physical map of Magnaporthe oryzae using genomic position-ready SSR markers  

作　　者：FENG ShuJie MA JunHong LIN Fei WANG Ling PAN QingHua 

机构地区：[1]Laboratory of Plant Resistance and Genetics, College of Natural Resources and Environmental Science, South China Agricultural University, Guangzhou 510642, China [2]Laboratory of Plant Protection, College of Horticulture, South China Agricultural University, Guangzhou 510642, China

出　　处：《Chinese Science Bulletin》2007年第24期3346-3354,共9页

基　　金：Supported by the National Basic Research Program of China (Grant No. 2006CB100206);Program for Changjiang Scholars and Innovation Research Team in University (Grant No. IRT0448) ;the Natural Science Foundation of Guang-dong Province (Grant No. 039254; 07300695)

摘　　要：Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat (SSR)-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were locatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr (avirulence) genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolu- tionally related pathogens.Magnaporthe oryzae is a model for plant pathogenic filamentous fungi. We have assembled a simple sequence repeat （SSR）-based physical map of the species, using in silico sequence data. A set of 120 SSR markers was developed from the genomic sequence of the reference isolate 70-15. These markers were readily amplified from the genomic DNA of other isolates, and high levels of allelic variation characterised the parental isolates of the two crosses tested. All the markers were Iocatable to one of the seven M. oryzae chromosomes. An SSR-based physical in silico map was constructed, and pre-existing SSR and RFLP loci were integrated into the map, along with 23 Avr（avirulence） genes and two other genes of importance to the plant/pathogen interaction. This map provides a platform for population genetics and functional genomics studies in the model pathogen, and even in other evolu- tionally related pathogens.

关 键 词：巨水仙碱 电子物理地图 单频重复 植物 病原体 

分 类 号：R284[医药卫生—中药学]