四肽化合物CMS030抗移植排斥作用的实验研究  

Study on the suppressive effects of CMS030 tetramer on allograft rejection in mice

作　　者：赵岚[1] 陆融 王松[1] 傅正[1] 李秋丽[1] 王莉[1] 吕军强[1] 马睿[1] 徐琼[1] 姚智[1] 

机构地区：[1]天津医科大学免疫学教研室,天津300070 [2]深圳康哲药业有限公司,深圳518029

出　　处：《中国免疫学杂志》2007年第9期824-828,共5页Chinese Journal of Immunology

基　　金：国家"863"项目(2004AA2Z3170;2005AA2Z3D40);国家"973"项目(2003CCA04300);教育部重点项目(03007)资助

摘　　要：目的:CMS030是从脾脏提取的四肽化合物,本研究观察CMS030对小鼠皮肤和心肌移植排斥反应的影响。方法:通过体外T淋巴细胞转化实验和混合淋巴细胞反应(MLR)实验,观察CMS030在体外的免疫抑制作用,建立小鼠皮肤移植和心肌移植模型,观察CMS030对小鼠同种移植物存活时间的影响,观察CMS030对移植受体小鼠T淋巴细胞转化和脾细胞分泌IL-2的影响。结果:CMS030在体外能够抑制T淋巴细胞转化和MLR,在体内可以明显延长小鼠皮肤移植物和心肌移植物存活时间,抑制皮肤移植受体小鼠T淋巴细胞转化能力和脾细胞分泌IL-2水平。结论:CMS030通过抑制T淋巴细胞分泌IL-2,抑制T淋巴细胞功能,有效拮抗移植排斥反应。Objective：To investigate the effects of CMS030 on skin and cardiac allograft rejection in mice. Methods：The effects of CMS030 in vitro were studied by ConA induced T ceII proliferation and mixed Iymphocyte reaction （MLR）. The tail skin grafts of BALB/c mice were placed in wound bed on the back of C57BL/6 mice, and the cardiac allografts of new-born C57BL/6 mice were transplanted into the posterior of BALB/c mice. The effects of CMS030 on the survival time of the grafts were observed. ConA-in- duced lymphocyte proliferation and the IL-2 of the mice were examined. Results：The inhibition effects of CMS030 on T cells were dis- played in vitro and the survival time of grafts was significantly prolonged by CMS030 in vivo. ConA-induced lymphocyte proliferation and the IL-2 secretion of the mice were both reduced by CMS030. Conclusion. CMS030 can inhibit IL-2 secretion of T lymphocytes, and suppress allograft rejection efficiently.

关 键 词：CMS030 皮肤移植 心肌移植 IL-2 

分 类 号：R392.4[医药卫生—免疫学]