复灌Ⅰ号注射液对大鼠肢体缺血—再灌注骨骼肌氧化损伤的保护机制  

The Protective Mechanism of Fu Guan Injection Ⅰ on Oxidative Damage of Rat's Skeletal Muscle Caused by Limb Ischemic Reperfusion

作　　者：朱付平[1] 熊光仲[1] 范志英[1] 袁锋[1] 李艳艳[1] 陈丹[1] 龚志贤[2] 

机构地区：[1]中南大学湘雅二医院,湖南长沙410011 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医药导报》2007年第12期8-12,19,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:探讨复灌Ⅰ号注射液对大鼠肢体缺血—再灌注骨骼肌氧化损伤的保护机制。方法:按文献[3]所报道的方法复制SD大鼠后肢缺血—再灌注模型。对照组动物不给药物;缺血再灌注组(IR组)于造成缺血前12 h、造成缺血时和再灌注时于腹腔内各注射生理盐水1 ml;复灌Ⅰ号药物干预组(Ⅰ-IR组)于造成缺血前12 h、缺血时和再灌注时腹腔各注射复灌Ⅰ号注射液1 ml。各组大鼠于相应时间点切取约1000 mg右侧腓肠肌中部肌肉,用于骨骼肌MDA、SOD、MPO的检测;切取左后肢腓肠肌行干湿重比值的检查;大鼠下腔静脉穿刺取血,取血清MDA、SOD、MPO、NO检测。结果:⑴MDA的检测结果:IR与Ⅰ-IR各组MDA含量均较对照组高,差异有统计学意义(P<0.05),且在8 h内随着时间的延长而逐渐增高。复灌Ⅰ号干预各组在相应时间点均较IR组低,各组间比较,差异有统计学意义(P<0.05)。⑵SOD的检测结果:IR与Ⅰ-IR各组血清和骨骼肌SOD值均较对照组低,差异有统计学意义(P<0.05),在8 h内其含量随时间的延长而逐渐降低。⑶MPO的检测结果:IR与Ⅰ-IR各组血清和骨骼肌MPO值均较对照组高,差异有统计学意义(P<0.05),在8 h内随时间的延长其含量逐渐升高,而Ⅰ-IR各组在相应时间点上均较IR组低,差异均有统计学意义(P<0.05)。⑷骨骼肌干湿重的检测结果:IR与Ⅰ-IR各组骨骼肌水肿明显,8 h内水肿逐渐增加,与对照组相比,差异有统计学意义(P<0.05)。⑸血清NO的检测结果:在再灌注后2 h时最高,8 h内随着时间的延长而逐渐下降,IR与Ⅰ-IR各组NO含量均较对照组高,差异有统计学意义(P<0.05)。结论:复灌Ⅰ号注射液可减少氧自由基的产生、减轻SOD降低的程度、降低PMN的游出数量、降低血清中NO的含量、减轻组织水肿程度。Objective： To study the protective mechanism of Fu Guan Injection I on oxidative damage of rat＇s skeletal muscle caused by limb ischemic reperfusion. Methods： The rats in control group were not blocked blood flow in posterior limbs using untied rubber band. The rats in ischemic reperfusion group （IR group） and Fu Guan injection I intervention group（ Ⅰ -IR group） were duplicated rats＇posterior limbs ischemic reperfusion models by the method suggested by Iiterature three. No drug was given before sacrifice of rats in control group. 1 ml normal sodium was intraperitoneal injected at the time 12 hours before ischemic, ischemic time and repcrfusion time in the 1R group. 1 ml Fu Guan Injection I was intraperitoneal injected at the time 12 hours before ischemic, ischemic time and reperfusion time in the IR group. Each group, 1000 mg right gastrocnemius muscle was obtained to tissue homogenate for skeletal muscle＇s MDA,SOD, MPO test. Some posterior left gastrocnemius muscle was obtained to dry -humid weight ratio test. Then some blood was obtained from inferior caval vein to blood serum MDA,SOD,MPO,NO test. Results： （1）Results of MDA test： The contents of MDA in IR and Ⅰ -IR groups were higher than control group, there were statistical difference（ P 〈0.05 ）. Moreover, the content of MDA was gradual higher with time passed within 8 hours after reperfusion. The content in Ⅰ - IR - 2, Ⅰ - IR - 4, Ⅰ - IR - 8 was lower than corresponding groups of IR group, there were statistical difference between IR group and I - IR group（P 〈0.05）. （2） Resuhs of SOD test： The content of SOD in IR and Ⅰ -IR groups was lower than control group, there were statistical difference（P 〈 0. 01）. Moreover, the content of SOD was gradual lower with time passed within 8 hours after reperfusion. There were statistical difference between IR group and Ⅰ - IR group（P 〈 0.05 ）. （3） Results of PMO test： The content of MPO in IR and Ⅰ - IR groups was higher than control group, ther

关 键 词：复灌Ⅰ号注射液 肢体缺血-再灌注损伤 骨骼肌 治疗 

分 类 号：R285.5[医药卫生—中药学]