卡维地洛与庚醇减少低氧再供氧心肌细胞凋亡及坏死的研究  

Effects of Carvedilol and Heptanol On Myocardial Cells Apoptosis and Necrosis in Ischemic Reperfusion

作　　者：曾玉杰[1] 冯义柏[2] 于世龙[2] 柯元南[1] 王勇[1] 李宪伦[1] 朱丹丹[1] 李志远[1] 

机构地区：[1]北京中日友好医院心内科,100029 [2]华中科技大学同济医学院附属协和医院心内科,武汉430022

出　　处：《医药导报》2007年第7期723-725,共3页Herald of Medicine

摘　　要：目的研究卡维地洛(carvedilol,CVD)、庚醇(heptanol,HT)对不同时间低氧再供氧心肌细胞坏死、凋亡的影响。方法利用锥虫蓝染色技术和流式细胞仪分别观察低氧后不同时间再供氧细胞坏死、凋亡情况,观察CVD、HT对其的影响。结果对照组(未给药)正常和低氧30 min时凋亡指数差异无显著性(P>0.05),再供氧1 h凋亡指数为27.4%,与正常时比较差异有极显著性(P<0.01)。坏死的心肌细胞在再供氧2 h后差异有极显著性(P<0.01)。运用CVD、HT后凋亡指数在再灌注1 h时分别较对照组减少了55.11%和21.53%。结论HT和CVD通过不同的作用可以减少低氧再供氧心肌细胞的死亡和凋亡。Objective To investigate the effects of carvedilol （CVD） and heptanol （HT） on myocytes necrosis and apoptosis in ischemic repeffusion at different time periods Methods The necrosis and apoptosis of myecytes inducedby reperfusion after different time periods of ischemia were observed by trypan blue staining and flow cytometry. The effects of CVD and HT on myecytes necrosis and apoptosis were studied. Results The indexes of apoptosis showed no significant difference between normal cells and ischemic cells for 30 rain （ P 〉 0.05 ）, the apoptotic indexes were 27.4% in the cell reperfusion after 1 h of ischemia （ P 〈 0.01 ） as compared with the normal control , and cellular necrosis increased very significantly in cell reperfusion after 2 h of ischemia. In CVD group and HT group, the apoptotic indexes reduced by 55. 11% and 21.53% , respectively as compared with the control group（ no drug administration）. Conclusion HT,CVD reduce the indexes of the cardiac myecyte apoptosis and necrosis.

关 键 词：卡维地洛 庚醇 心肌细胞凋亡 

分 类 号：R971.94[医药卫生—药品] R965[医药卫生—药学]