不同粒径纳米血竭和普通血竭对肿瘤细胞的体外效应  

The Effects of Sanguis Draxonis Nanoparticles on Five Tumor Cell Lines in Vitro

作　　者：朱慧芬[1] 杨道锋[2] 王敏[1] 刘静[1] 文雪[1] 张悦[1] 冯玮[1] 龚非力[1] 沈关心[1] 

机构地区：[1]华中科技大学同济医学院免疫学研究所,武汉430030 [2]华中科技大学同济医学院附属同济医院感染科,武汉430030

出　　处：《医药导报》2007年第7期744-747,共4页Herald of Medicine

摘　　要：目的观察不同粒径血竭纳米颗粒在体外对宫颈癌细胞株Hela、肺癌细胞株A549、肝癌细胞株SMMC7721、卵巢癌细胞株A2780和粒细胞白血病细胞株HL-60的杀伤作用,并探讨其作用机制。方法以不同浓度和不同粒径纳米血竭分别加入培养的上述肿瘤细胞株中,流式细胞仪检测各种细胞的死亡率、凋亡率及其细胞周期,并与普通血竭比较。结果粒径为171,167和23.8 nm的纳米血竭在浓度3.9～31.2 mg.L-1范围对细胞株HL-60的杀伤百分率明显高于普通血竭,其主要作用机制不是诱导细胞凋亡,细胞周期测定表明血竭纳米颗粒主要将HL-60细胞阻滞在G0/G1期。纳米血竭对其他肿瘤细胞的作用与普通血竭差别不明显。结论粒径为171,167和23.8 nm的纳米血竭对肿瘤细胞株HL-60有明显的杀伤作用,其效率高于非纳米血竭。Objective To investigate the anti-tumor effects of Sanguis draxonis nanoparticles on cervix cancer cell line Hela, pulmonary carcinoma cell line A549, hepatocarcinoma cell line SMMC7721, ovarian cancer cell line A2780 and granulocytic leukemia cell line HL-60. Methods A consecutive concentrations and a various diameters of Sanguis draxonis nanoparticles were added into above tumor cell culture plates. Cell death rate, apoptosis rate and cell cycle were detected by flow cytometry. Sanguis draxonis common particle was employed as a control. Results The kill rates of Sanguis draxonis nanoparticles on HL-60 cells in 3.9 - 31.2 mg · L^-1 were higher than those of common Sanguis draxonis when their diameters were 171 nm, 167 nm and 23.8 nm. The cell apoptosis was not a principal mechanism of their anti-tumor effects. Cell cycle assay demonstrated that most HL-60 cells were stopped in G0/G1 phase. Conclusion Sanguis draxonis nanoparticles in 171 nm, 167 nm and 23.8 nm diameters can kill HL-60 cells effectively with higher effective rate than that of the common Sangius draxonis.

关 键 词：血竭 纳米 肿瘤细胞 杀瘤效应 凋亡 

分 类 号：R282.74[医药卫生—中药学]