高牵张促进烧伤血清复合缺氧条件下心肌细胞p38磷酸化  

Effects of high strectch on the phosphorylation of p38 in the cardiocyte under the circumstances of burn sera and hypoxia

作　　者：范鹏举[1] 黄跃生[2] 黄晓元[1] 郑军[3] 

机构地区：[1]中南大学湘雅医院烧伤整形科,长沙市410008 [2]第三军医大学西南医院烧伤研究所,创伤,烧伤,复合伤国家重点实验室,重庆市400038 [3]南华大学附属第一医院烧伤科,湖南省衡阳市421001

出　　处：《实用医学杂志》2007年第24期3828-3830,共3页The Journal of Practical Medicine

基　　金：国家重点基础研究发展规划资助项目("973"项目)(编号:G1999054205);国家杰出青年科学基金资助项目(编号:30125040)

摘　　要：目的:探讨高负荷对烧伤血清复合缺氧条件下心肌细胞p38表达、磷酸化的影响。方法:采用原代培养的乳鼠心肌细胞,分别给予正常血清(SN组)、烧伤血清+缺氧(SH组)、烧伤血清+缺氧+10%轴向静态牵张(SHS组)3种处理,采用Western blotting和免疫荧光检测磷酸化p38(p-p38)于心肌细胞内的表达情况。结果:SHS组较SH组p38表达、磷酸化增强,且磷酸化高峰提前,免疫荧光分析证实SH组、SHS组心肌细胞内p-p38水平明显提高。结论:高牵张上调烧伤血清复合缺氧条件下心肌细胞p38的表达和磷酸化水平,后者可能是高负荷对心肌细胞损伤的主要方式。Objective To investigate the effects of high stretch on the expression and phosphorylation of p38 in the cardiocyte under the circumstance of burn sera combined with hypoxia. Method Ventricular cardiac myocytes isolated from neonatal rats were employed in this study. The cells were stimulated by sera of normal rat（SN group）, burn sera and hypoxia（SH group）, burn sera and hypoxia and 10% axial static stretch （SHS group）, respectively, p38 expression and phosphorylation （p-p38） in the cardiomyocyte were determined by Western blotting and immunofluorescence. Results The expression and phosphorylation of p38 in SHS group more increased than that in SH group, and the peak of its phosphorylation in SHS group was in ahead of SH group. Immunoflurescence＇s result demonstrated that the level of p-p38 of the cardiocyte in SH group and SHS group raised remarkably. Conclusion High stretch raises the expression and phosphorylation of p38 in the cardiocyte under the circumstance of burn sera combined with hypoxia, of which the latler may be the major way to damage the cardiocytes.

关 键 词：烧伤 心肌 P38丝裂原活化蛋白激酶类 

分 类 号：R644[医药卫生—外科学]