银纳米敏化铽-诺氟沙星荧光和二级散射的研究与应用  

Nanosilver Sensitized Fluorescence and Second-Order Scattering of Tb(Ⅲ)-Norfloxacin and Its Application

作　　者：杨志洁[1] 赵慧春[1] 丁芬[1] 李爱云[1] 王晓莉[1] 

机构地区：[1]北京师范大学化学学院

出　　处：《光谱学与光谱分析》2007年第12期2534-2537,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20331010)资助

摘　　要：铽（Ⅲ）（Tb3＋）与诺氟沙星能生成二元配合物,在272 nm紫外光照射下配合物中的配体诺氟沙星能吸收能量并将其能量转移给稀土铽（Ⅲ）离子,产生铽（Ⅲ）离子的特征荧光峰,最大峰位于545 nm。而配合物的二级散射峰也恰好在545 nm。当一定浓度和一定大小颗粒的银纳米粒子加入到二元体系中时,不仅增强了铽（Ⅲ）与诺氟沙星配合物分子间的能量传递,同时也增大了体系的二级散射光强度,使得体系在545nm处的总发射光强度大大增加,且体系的发光强度与诺氟沙星的浓度在一定浓度范围内成正比。据此建立了一种新的诺氟沙星的分析方法。实验研究了银纳米粒子-铽（Ⅲ）-诺氟沙星体系测定诺氟沙星的最佳实验条件,线性范围为6.0×10^-9-1.0×10^-5mol·L^1,检出限为4.4×10^-9mol·L^-1。由于产生的发射光谱是长波区的锐线光谱,该方法干扰少、灵敏度高、选择性好,用于测定胶囊和眼药水中诺氟沙星的含量,其回收率在93.2%-102.0%。Norfloxacin （NFLX） can form complex with Tb（Ⅲ） ion, and the intramolecular energy transfer from NFLX to Tb （Ⅲ） takes place when excited. And thus the characteristic fluorescence of Th（Ⅲ） ion is enhanced and the maximum fluorescence peak locates at 545 nrn. The second-order scattering （SOS） peak at 545 nm also appears for the Th（ Ⅲ ）-NFLX complex with the excitation wavelength of 272 nm. When the silver nanoparticles were added to the Tb（Ⅲ）-NFLX binary system, the luminescence intensity at 545 nm greatly increased. And the relative intensity is proportional to the amount of NFLX. Based on this phenomenon, a novel method for the determination of NFLX has been developed by using a common spectrofluorometer to measure the intensity of fluorescence and SOS. The calibration graphs for NFLX are linear in the range of 6.0×10^-9-1.0×10^-5mol·L^1 , and the detection limit is 4. 4× 10^-9 mol·L^1. This method was applied satisfactorily to the determination of NFLX in capsule and eye drop samples. The experimental results showed that it is the certain size and certain concentration of silver nanoparticles that can greatly enhance the fluorescence-SOS intensity.

关 键 词：银纳米粒子 二级散射 铽 诺氟沙星 荧光 

分 类 号：O657.3[理学—分析化学]