HPLC-ELSD法测定盾叶薯蓣中薯蓣皂苷的含量  

HPLC-ELSD determination of dioscin in Rhizoma Dioscoreae Zingiberensis

作　　者：王光忠[1] 张明[1] 刘焱文[1] 

机构地区：[1]湖北中医学院中药资源与中药化学省级重点实验室,武汉430061

出　　处：《药物分析杂志》2007年第12期1906-1908,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：湖北省教育厅重点项目(D200516012)

摘　　要：目的:建立盾叶薯蓣中薯蓣皂苷的含量测定方法。方法:采用高效液相色谱-蒸发光散射检测法(HPLC—ELSD),Allti-ma C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(52:48),流速1.0 mL·min^(-1);蒸发光散射检测器参数:漂移管温度40℃,气压3.5 kPa。结果:薯蓣皂苷在0.24～4.80μg(r=0.9995)范围内线性关系良好,平均回收率为97.9%(RSD=1.1%,n=6)。结论:该方法简便、快速、灵敏、准确、重现性好,可作为盾叶薯蓣的质量控制方法。Objective：To develop a method for the determination of dioscin in Rhizoma Dioscoreae Zingiberensis. Methods：HPLC -ELSD was used in the quantitative analysis with Alhima C lschromatography column（4.6 mm× 250 mm,5 μm） and acetonitrile- water（52：48） as a mobile phase. The flow rate of mobile phase was 1.0 mL . min^-1. The drifting tube temperature of the detector was 40 ℃. The pressure of pure air was 3.5 kPa. Results：The linear range for dioscin was 0. 24 - 4. 80 μg（ r = 0. 9995 ）, the average recoveries was 97.9% （ RSD = 1.1% , n = 6）. Conclusion： This method is simple, rapid, sensitive, accurate, reproducible and can be trol method of Rhizoma Dioscoreae Zingiberensis.

关 键 词：盾叶薯蓣 高效液相色谱-蒸发光散射检测法 薯蓣皂苷 

分 类 号：R917[医药卫生—药物分析学]