HPLC法测定叶下珠胶囊中诃里拉京的含量  

HPLC determination of corilagin in Yexiazhu capsules

作　　者：董媛 明全忠 李忠琼 张颖君 

机构地区：[1]云南省食品药品检验所,昆明650011 [2]昆明植物研究所,昆明650011

出　　处：《药物分析杂志》2007年第12期1911-1912,共2页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱法分离和测定叶下珠胶囊中诃里拉京。方法:采用 Alltech C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%磷酸溶液(7.5:92.5)为流动相,流速为1.0 mL·min^(-1),柱温为25℃,检测波长为216 nm。以外标法测定了叶下珠胶囊中诃里拉京的含量。结果:诃里拉京在0.08～20.49μg(r=0.9999)的范围内呈现出良好的线性关系,诃里拉京的平均回收率(n=9)为98.8%,RSD 为0.8%。结论:本方法简便易行,结果准确可靠,可用于叶下珠胶囊的质量控制。Objective： To establish a method for the determination of corilagin in Yexiazhu capsules by HPLC. Methods：The chromatographic conditions were as follows ：Alltech C 18 column （250 mm × 4.6 mm,5 μm） with the mobile phase of acetonitrile -0. 2% phosphoric acid（7.5： 92. 5） at the flow rate of 1.0 mL . min^-1 and UV detection wavelength was at 216 nm, column temperature was 25 ℃. Results： The calibration curve was linear in the range of 0.08 - 20. 49 μg for corilagin（ r = 0. 9999 ）. The average recovery was 98.8% （ n = 9 ）, RSD was 0. 8%. Conclusion：The method is accurate and reliable for the quality control of this preparation.

关 键 词：叶下珠胶囊 诃里拉京 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]