免疫荧光及双抗体夹心ELISA在人狂犬病检测中的应用  

Application of Immunofluorescence and Sandwich ELISA with Double-antibodies in Detection of Human Rabies

作　　者：孙来晶[1] 徐葛林[2] 王华林[3] 吴杰[2] 胡志红[3] 周亦武[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]武汉生物制品研究所基因工程室,湖北武汉430060 [3]中国科学院武汉病毒研究所病毒学国家重点实验室,湖北武汉430071

出　　处：《法医学杂志》2007年第6期411-413,共3页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30570629)

摘　　要：目的探讨免疫荧光和双抗体夹心ELISA在人狂犬病检测中的应用。方法4例临床诊断狂犬病死亡患者的大脑、小脑、脑干、海马,分别于冰箱和甲醛固定后保存,用上述方法检测。感染狂犬病毒CVS株的鼠脑组织和急性胰腺炎死者脑组织分别作为阳性和阴性对照。结果-70℃保存的人脑和阳性对照狂犬病毒均为阳性,甲醛固定的人脑和阴性对照均为阴性。结论免疫荧光和双抗体夹心ELISA可用于人狂犬病检测,但脑组织要低温保存,不能用甲醛固定。Objective To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies. Methods The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 ℃ or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute panereatitis was used as a negative control. Results Rabies virus was detected in the tissues kept in freezer at -70℃ and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control. Conclusion Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.

关 键 词：人狂犬病 法医学鉴定 免疫荧光 双抗体夹心ELISA 

分 类 号：DF795.1[医药卫生—法医学]