二醋吗啡对大鼠心肌细胞游离Ca^(2+)浓度的作用  

The Effects of Heroin on Intracellular Free Ca^(2+) of Rat Myocardium

作　　者：刘小山[1] 吴敬东[2] 蒿自睿[1] 刘水平[1] 李朝晖[1] 李海霞[1] 陈玉川[1] 

机构地区：[1]中山大学中山医学院法医系,广东广州510080 [2]中山市公安局石歧分局,广东中山528403

出　　处：《法医学杂志》2007年第6期424-427,共4页Journal of Forensic Medicine

摘　　要：目的观察二醋吗啡对心肌细胞内游离Ca2+浓度的作用。方法取自1～3d的SD大鼠培养的心肌细胞,用荧光探针Fluo-3/AM负载心肌细胞,不同浓度及不同剂量的二醋吗啡作用心肌细胞,激光共聚焦显微镜下检测Ca2+的变化。结果不同剂量及浓度的二醋吗啡对心肌细胞内游离Ca2+浓度有不同作用,一定浓度的二醋吗啡使心肌细胞内游离Ca2+浓度呈剂量依赖性的升高、短时间内心肌细胞[Ca2+]i荧光强度增强,并产生[Ca2+]i峰。结论探索出二醋吗啡导致心肌细胞内Ca2+变化的有效浓度,为进一步深入研究打下了基础。Objective To observe the effects of heroin Methods The effects of heroin on intracellular free on intracellular free Ca^2＋ in rat myocardium. Ca^2 ＋ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca^2＋ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope. Results Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca^2＋ coneentrations, with a dosage dependent short linear increase in the fluorescence intensity （i.e., Ca^2＋ concentration） leading to [Ca^2＋]i peak. Conclusion Heroin could affect eoncentrations of [Ca^2＋ ]i in myoeardium and its dosage related effect needs further investigation.

关 键 词：二醋吗啡 心肌细胞 游离CA^2+ 

分 类 号：DF795.1[医药卫生—法医学]