对链脲佐菌素诱导的糖尿病大鼠壁细胞和G细胞的体视学研究  

A stereological study on the parietal cells and the gastrin cells of the streptozotocin induced diabetic rats

作　　者：赵超[1,2] 叶光福[1,2] 王庆堂[1,2] 秦光明[1,2] 李肇春 张敏海[1,2] 张亦农 汪薇曦[1,2] 

机构地区：[1]广东省中山市博爱医院内科 [2]武汉同济医科大学

出　　处：《中国糖尿病杂志》1997年第3期156-159,共4页Chinese Journal of Diabetes

摘　　要：采用ＨＥ染色和免疫组化方法结合生物体视学技术，对链脲佐菌素诱导的糖尿病大鼠在胃底腺的壁细胞和幽门部胃粘膜的Ｇ细胞进行立体计量研究。结果显示：糖尿病状态的早期，壁细胞和Ｇ细胞的体积均明显增大，数量却显著减少。根据正常情况下壁细胞和Ｇ细胞的细胞动力学变化、胃泌素的生物学作用和上述实验结果，认为大鼠胃底腺峡部的干细胞向壁细胞分化成熟的功能及Ｇ细胞的分裂增殖活动，在胰岛素缺乏的情况下受到一定程度的抑制，而这种功能的抑制是糖尿病状态下易出现胃粘膜萎缩、胃酸分泌减少和胃轻瘫的重要原因之一。The stereological survey on the parietal cells in the gastric oxyntic glands and the gastrin cells in the gastric pyloric mucosa of the streptozotocin induced diabetic rats was performed using combined HE staining and gastrin's immunocytochemistry by Weibel's stereological methods.The results showed that the sizes of parietal cells and gastrin cells significantly increased,but the numbers of these cells significantly reduced in the early stage of diabetes. According to the dynamics of parietal cells and gastrin cells under normal states, biological effects of gastrin and the above experimental results,we believe that the functions of differentiation and maturation of the parietal cells and the splitting and proliferation of the gastrin cells from the stem cells in isthmus of the oxyntic glands might be partly inhibited because of the deficiency of insulin. The inhibition of these functions might play an important role in the pathogenesis of atrophic gastritis, decrease of gastric acid secretion and partly attribute to gastroparesis in diabetes mellitus.

关 键 词：糖尿病 链脲佐菌素 壁细胞 G细胞 体视学 

分 类 号：R587.102[医药卫生—内分泌]