HPLC法测定利通淋胶囊中总黄酮醇苷的含量  

Determination of total flavonol in Litonglin capsules by HPLC

作　　者：李荣辉[1] 

机构地区：[1]晋江市疾病预防控制中心,晋江362200

出　　处：《海峡药学》2007年第12期48-49,共2页Strait Pharmaceutical Journal

摘　　要：目的建立HPLC法测定利通淋胶囊中总黄酮醇苷含量。方法采用KromasilC18（4．6×250mm，5μm）色谱柱；流动相：甲醇-0．4％磷酸溶液（55：45）；流速：1．0mL·min^-1；测定检测波长为360nm。结果槲皮素在浓度4、067μg·mL^-1-40．67μg·mL^-1范围内线性关系良好（r=0．9998）；山奈酚在浓度0．871μg·mL^-1～8．71μg·mL。范围内线性关系良好（r=0．9998）；异鼠李素在浓度0．616μg·mL^-1-6．161g·mL^-1范围内线性关系良好（r=0．9996）。结论本方法简便、准确、专属性强，可测定利通淋胶囊中总黄酮醇苷的含量。OBJECTIVE To develop a HPLC method for the determination of total flavonol in Litonglin capsules by HPLC. METHODS The chromatographic analysis was performed on Kromasil C18 （4. 6 × 250mm,5 μm）column. The mobile phase consisted of methonal - 0.4% phosphoric acid （ 55：45 ）. The flow rate was 1.0mL · min ^-1. The detection wavelength was at 360nm. RESULTS Quercetin ： A good linearity was obtained over the range of 4. 067μg · mL^-1 ~40. 67μg · mL^-1（ r =0. 9998）. Kaempferol： A good linearity was obtained over the range of 0. 871μg · mL^-1 - 8.71μg · mL^-1 （ r = 0. 9998 ）. Isorhamnetin： A good linearity was obtained over the range of 0. 616μg · mL^- 1 ~ 6. 16μg · mL^- 1 （ r = 0. 9996 ）. CONCLUSION The method was simple, accurate, specific and could be used for the quality control of quercetin, kaempferol and isorhamnetin in crude saponins of Litonglin capsules.

关 键 词：利通淋胶囊 HPLC法 总黄酮醇苷 含量 

分 类 号：R927.2[医药卫生—药学]