利用RAPD标记分析卧龙自然保护区不同海拔沙棘种群的遗传变异(英文)  

Genetic Variation of Hippophae rhamnoides Populations at Different Altitudes in the Wolong Nature Reserve Based on RAPDs

作　　者：赵春芳[1] 陈国娟[1] 王芋华[1] KORPELAINEN Helena 李春阳[1] 

机构地区：[1]中国科学院成都生物研究所 [2]赫尔辛基大学应用生物学系,芬兰赫尔辛基fi00014

出　　处：《应用与环境生物学报》2007年第6期753-758,共6页Chinese Journal of Applied and Environmental Biology

基　　金：Supported by the Outstanding Young Scientist Program of the NationalNatural Science Foundation of China ( No 30525036);the ChinaNational Key Program of the International Cooperation for Science and Technology (No 2005DFA30620)

摘　　要：从1800m到3400m五个海拔连续取样,用RAPD分子标记研究了卧龙自然保护区中国沙棘种群的遗传结构和遗传变异.用11条寡核苷酸引物,扩增得到151个重复性好的位点,其中143个多态位点,多态率达94.7%.在5个沙棘种群中,总遗传多样性值(HT)为0.289,B种群内的遗传多样性值为0.315,这完全符合沙棘这种多年生、远交的木本植物高遗传变异的特性.5个种群内遗传多样性随海拔升高呈低—高—低变异趋势,在2200m海拔处的B种群遗传多样性达最大值0.315,3400m海拔处的E种群则表现最小,仅0.098.5个种群间的遗传分化值GST=0.406,也即是说有40.6%的遗传变异存在于种群间,59.4%存在种群内.1800m海拔处的A种群与其他种群的明显分离是造成种群间遗传分化大的原因.UPGMA聚类图和PCoA散点图分别进一步确证了5个种群间关系和所有个体间的关系.最后,经过Mantel检测,遗传距离与海拔表现了明显的相关性(r=0.646,P=0.011).To estimate genetic variation and genetic structure of Hippophae rhamnoides ssp. sinensis at different ahitudes, five natural populations in the Wolong Nature Reserve at altitudes ranging from 1 800 to 3 400 m above sea level （ a. s. 1. ） were surveyed using random amplified polymorphie DNA markers （RAPDs）. Based on 11 decamer primers, a total of 151 reproducible DNA loci were yielded, and 143 of them were polymorphic, accounting for 94. 7%. Among the five populations investigated, the total gene diversity （Hv） and gene diversity within population B equaled 0. 289 and 0. 315, respectively, which are modest for a subspecies of H. rhamnoides that is an outcrossed and long-lived woody plant. The amount of genetic variation among populations varied from 0.098 within population E （3 400 m a. s. 1. ） to 0. 315 within population B （2 200 m a. s. 1. ）. The coefficient of gene differentiation （GST） among populations equaled 0. 406, and revealed that 40.6% of the genetic variance existed among populations and 59.4% within populations. The population A （ 1 800 m a. s. 1. ） differed greatly from the other four populations, which contributes to high genetic differentiation. A UPGMA cluster analysis and principal coordinate analyses based on Nei＇s genetic distances fllrther corroboraled lhe relalionships among the five populalions and all lhe sampling individuals, respectively. Mantel tests detected a significant correlation between genetic distances and ahilndinal gradients （r =0.646, P--0.011）.

关 键 词：海拔梯度 遗传分化 遗传变异 沙棘 RAPD 

分 类 号：S793.6[农业科学—林木遗传育种] Q943[农业科学—林学]