计算机辅助精子分析系统在大鼠精子运动能力测定中的应用  

Application of Computer Assisted Sperm Analysis System in Detection of Rat Sperm Motility

作　　者：韦凌娅[1,2] 吕中明[1] 王民生[1] 

机构地区：[1]江苏省疾病预防控制中心毒理实验室 [2]东南大学,公共卫生学院,江苏南京210009

出　　处：《环境与职业医学》2007年第6期596-598,共3页Journal of Environmental and Occupational Medicine

基　　金：江苏省疾病预防控制中心青年科研基金(编号:JSQ200509)

摘　　要：[目的]应用计算机辅助精子分析(computer assisted sperm analysis,CASA)技术研究氯化镉(CdCl_2)不同时间染毒对大鼠成熟精子运动能力的影响。[方法]用扩散法收集大鼠附睾尾成熟精子制成精子悬液,分8组,CdCl_2染毒浓度分别为0,0.5,1.0,2.0,4.0,8.0,16.0,32.0mg/L,每组6个平行样,染毒30min、1h、2h、4h后用CASA仪测定反映大鼠精子运动能力及运动方式的各项参数。[结果]CdCl_2染毒30min后,染毒组精子平均路径速度(VAP)、曲线运动速度(VCL)、直线运动速度(VSL)、直线性(LIN)和精子头侧摆幅度(ALH)等参数与对照组相比差异有统计学意义(P<0.05),鞭打频率(BCF)、前向性(STR)在1.0～32.0mg/L的染毒范围内差异有统计学意义(P<0.05),VAP值随着染毒浓度增加有降低的趋势(r=-0.348,P<0.05)。随着CdCl_2染毒浓度的增加和时间的延长,精子运动能力下降,呈现剂量-效应和时间-效应关系。[结论]体外CdCl_2染毒可以引起成熟精子的运动能力下降。[ Objective ] To study the effect of cadmium chloride （ CdCl2 ） on rat sperm motility in vitro with computer assisted sperm analysis system （ CASA ）. [ Methods ] The suspension of spermatozoa was obtained from cauda epididymides using ＂diffusion＂ method. Different doses of cadmium chloride（ 0, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, 32.0mg/L ）were administrated to spermatozoa suspension. Sperm motility were evaluated with CASA after 30min, 1 h, 2h, and 4h of exposure. [ Results ] After 30 min exposure in CdCl2, the indices such as VAP, VCL, VSL, LIN and ALH were significantly decreased （ P 〈 0.05 ）, BCF and STR were decreased significantly in the groups of exposed to 1.0-32.0mg/L CdCl2. VAP showed relationship with the dose （ r=-0.348, P 〈 0.05 ）. With the increasing of dose and the exposure continuance of cadmium chloride, sperm motility decreased significantly. [ Gonclusion ] Cadmium chloride could result in toxic effects on motility of sperms.

关 键 词：氯化镉 大鼠 精子 计算机辅助精子分析 

分 类 号：R114[医药卫生—卫生毒理学]