采用PCR技术对绒毛和羊水巨细胞病毒感染研究的比较分析  

Comparative analysis of studying human cytomegalovirus infection in villi and amniotic fluids by PCR method

作　　者：李钰[1] 张贵寅[1] 李敏[1] 吕航[1] 王霞[1] 

机构地区：[1]哈尔滨医科大学生物教研室,150086

出　　处：《中国优生与遗传杂志》1997年第4期19-20,16,共3页Chinese Journal of Birth Health & Heredity

摘　　要：本文采用PCR方法检测了早孕及孕中期羊水人巨细胞病毒（简称HCMV）的感染情况。孕早期101例绒毛的HCMV感染87例（87．1％），54例羊水标本经PCR检测发现HCMV阳性24例（44.4％）。进一步分析三个孕期的HCMV感染表明HCMV可以经胎盘传播给胎儿，同时胎盘有一定的屏障作用，而且母体及胎儿自身也有相当的保护能力。此研究结果证实PCR方法检测先天性HCMV感染敏感、快速，并具有特异性。We detected intrauterine human cytomegalovirus (HCMV) infectioin in the first trimester which was deteced in villi and midgestation which was detected in amniotic fluids. 87 cases of 101 cases in villi were positive (86.1%), 24cases of 54 amniotic fluids samples were positive (44.4%) which were identified by PCR mothod.Then we analyzed infection of HCMV in thhree trimesters. The result indicated that infection of HCMV in pregnant women may be transmited to fetus by placentae, and the placentae can defense HCMV infection in some degree. Also, there are comparatively ability to be protect against infection of HCMV in mother's body and fetus. Result of the study showed PCR method is a sensitive,speed, special technique to detect congenital HCMV infection.

关 键 词：巨细胞病毒感染 绒毛 羊水 聚合酶链反应 妊娠 

分 类 号：R714.251[医药卫生—妇产科学] R512.99[医药卫生—临床医学]