一种简易的泌尿生殖支原体的实验室药敏检测法的初步研究  

The preliminary study of a microplate antibiotics susceptibility test for the clinic laboratory of the urogenital Mycoplasmas

作　　者：尹少甫[1] 

机构地区：[1]兰州医学院微生物学教研室,730000

出　　处：《中国优生与遗传杂志》1997年第4期82-85,共4页Chinese Journal of Birth Health & Heredity

摘　　要：本文报告了用建立的微孔药敏法，分别以2mg／L、8mg／L、≥16mg／L时的抑制做为筛选对泌尿生殖道支原体的敏感、中度敏感、耐药的标准，筛选了8种对泌尿生殖道支原体有治疗作用的抗生素。结果表明：盐酸二甲胺四环素、强力霉素、氧氟沙星对人型和解脲支原体均有强的抑制作用；角沙霉素、洁霉素、克林霉素对人型支原体有抑制作用，而对解脲支原体多株耐药；红霉素对解脲支原体有中度抑制作用；四环素对两种支原体有中度抑制作用。本文也探讨了混合性支原体标本对微孔药敏的影响，虽然耐药株可掩盖敏感株的药敏测定水平，但可测出其共同药敏谱，有实际价值。认为这种简化的方法可做为常规泌尿生殖道支原体实验室的实用药敏方法。This article reports a simplified method of the microplate antibiotics susceptibility testing for urogenital Mycoplasma hominis (M. h) and Ureaplasma urealyticum(U. u), the standard of the judgement of level sensitive, median, resistant was the inhibition concentrations in 2 mg/L, 8 mg/L, ≥16 mg/L respectively, then 8 antibiotics had determinated. The results showed that Minocycline,Doxvcycline, Ofloxacin were active sensitive to M.h and U. u: Josamycin,Lincomycin, Clindamycin were mainly sensitive to M. h; Erythromycin was middle sensitive to U. u: the Tetracyclin was sensitive. median. or resistant to M. h and U. u, according to the strain. The speciments mixed of the two kinds of Mycoplasmas were also analysed.though resistant strain obscured the inhibition concentration of the sensitive strain, effected the susceptibility determination, it could indicate the common antibiotics spectrum of the two infectious strains, so this method can be used in clinic laboratory of the urogenital Mycoplasmas for the antibiotics selection.

关 键 词：泌尿系感染 生殖道支原体 实验室诊断 

分 类 号：R691.304[医药卫生—泌尿科学]