胶体金技术在鼠疫监测中的应用  

Application of Gold Immune-chromatographic Assay for Plague Surveillance

作　　者：汪立茂[1] 段勇军[2] 魏敏[1] 谢飞[3] 何前军[2] 罗志丹巴[2] 李光清[3] 宗康贵 马吉胜[4] 陈开华[4] 黎伦金[4] 张小兵[4] 邓兵 魏跃辉 周兴余[1] 

机构地区：[1]四川省疾病预防控制中心,四川成都610041 [2]甘孜州疾病预防控制中心 [3]石渠县疾病预防控制中心 [4]阿坝州疾病预防控制中心 [5]若尔盖县疾病预防控制中心

出　　处：《预防医学情报杂志》2008年第1期11-13,共3页Journal of Preventive Medicine Information

基　　金：国家"十五"科技攻关项目(项目编号2004BA718B07-01)

摘　　要：目的选择快速、敏感的鼠疫检测方法,为鼠疫防治提供科学依据。方法胶体金免疫层析法(GICA)与鼠疫间接血凝(IHA)和反向血凝(RIHA)同步检测鼠疫抗体/抗原。结果在四川省191份动物肝脾标本中,GICA检出阳性10份,RIHA阳性6份;检测动物血清标本417份,GICA与IHA均检出阳性13份。结论GICA简便快速,适合现场鼠疫监测。Objective To choose rapid-sensitive detection assay for the plague antibody/antigen so as to provide scientific basis for plague prevention and cure. Methods To detect the plague antibody/antigen with gold immune-chromatographic assay （GICA） , indirect hemagglutination assay（IHA） and reverse indirect hemaggluti - nation assay（RIHA） at the meantime. Results The positive samples were 10 with GICA and 6 with RIHA in 191 animal liver-spleen samples in Sichuan Province, The positive samples all were 13 with GICA and IHA in 417 animal serum samples. Conclusion GICA was proved to be a simple, rapid method for the detection plague antibody/antigen and quite suitable for the plague field surveillance.

关 键 词：胶体金 鼠疫 监测 免疫层析 

分 类 号：R516.8[医药卫生—内科学]