枳椇子总黄酮提取工艺的优化  

Optimization of Extracting Conditions of Zhijuzi by Alcohol

作　　者：文为[1] 张洪[1] 曾嵘[1] 

机构地区：[1]武汉大学人民医院药学部,430060

出　　处：《医药导报》2008年第1期77-79,共3页Herald of Medicine

基　　金：湖北省科技攻关项目(基金编号:2002AA301C61)

摘　　要：目的优选枳椇子提取物的最佳提取工艺。方法以提取物得率及提取物中总黄酮含量为检测指标,对提取溶剂进行筛选;通过考察小鼠肝匀浆中的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,确定最佳提取方法。同时采取正交设计法对最佳提取方法的提取工艺进行了考察。结果以75%乙醇为提取溶剂,采用热回流提取法,选择乙醇浓度为75%,提取时间2 h、乙醇加入量为8倍量为最佳提取工艺。结论优选得到的提取工艺合理、操作可行、质量可控。Objective To select the optimizal extracting conditions of zhijuzi by alcohol. Methods To screen the extracting dissolvent on the basis of extractive yield and total flavonoids. To ascertain the extracting conditions by investigating the SOD activity and the concertration of MDA in rats liver homogenate. The extracting process was determined by orthogonal design, Results The optimal extracting condition was heat reflux for 2 hours using 8 - fold 75% alcohol, Conclusion The extracting technology selected in this experiment is reasonable, pactical and conrtollable.

关 键 词：枳棋子 提取工艺 正交设计法 

分 类 号：R284.2[医药卫生—中药学] TQ460.6[医药卫生—中医学]