高效液相色谱法测定金果饮咽喉片中橙皮苷含量  

Determination of Hesperidin in Jinguoyinyanhou Tablet by HPLC

作　　者：胡婷霞[1] 祝明[2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江省药品检验所,浙江杭州310004

出　　处：《中国药业》2008年第1期10-11,共2页China Pharmaceuticals

摘　　要：目的建立金果饮咽喉片的橙皮苷含量测定方法。方法采用高效液相色谱(HPLC)法,色谱柱为ZorbaxSB-C18柱(250mm×4.6mm,5μm),柱温30℃,流动相为甲醇-6%醋酸溶液(38∶62),检测波长为283nm,流速为1.0mL/min。结果橙皮苷进样量在0.0285～0.9120μg范围内与峰面积线性关系良好,平均加样回收率为98.95%,RSD=0.7%。结论HPLC法操作简便、快速、准确,可有效控制金果饮咽喉片的质量。Objective To develop a HPLC method for the determination of hesperidin in Jinguoyinyanhou Tablet. Methods The analysis was carried out on a column of ZORBAX SB-C18（250 mm×4.6 mm,5 μm） at 30℃.The mobile phase consisted of methanol-6% acetic acid （38：62）,the flow rate was 1.0 mL/min, and the determination wavelength was at 283 nm. Results The linear range was from 0. 028 5- 0. 9210μg （ r = 1. 000 0） for hesperidin. The average recovery rate was 98.95% （ RSD = 0. 7% ）. Conclusion This method is simple, quick and accurate. It is available for quality control of Jinguoyinyanhou Tablet.

关 键 词：金果饮咽喉片 橙皮苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]