颈椎不稳黄韧带TGFβ1、BMP2表达的实验研究  

Expression of TGFβ1 and BMP-2 in the experimental ligamentum flavum of the unstable cervical spine

作　　者：张喜善[1] 蔡国栋[2] 李建民[1] 杨明峰[3] 

机构地区：[1]山东大学齐鲁医院骨科,济南250012 [2]泰山医学院附属医院骨科,山东泰安271000 [3]泰山医学院基础医学研究所,山东泰安271000

出　　处：《山东大学学报（医学版）》2007年第12期1253-1256,共4页Journal of Shandong University:Health Sciences

基　　金：山东省教育厅立项课题(项目编号:2006-2008J06L87)

摘　　要：目的探讨颈椎不稳对于黄韧带中转化生长因子β1(TGFβ1)、骨形态发生蛋白2(BMP2)基因表达的影响及其意义。方法采用逆转录聚合酶联反应技术(RT-PCR)对正常与颈椎不稳定动物模型的黄韧带中不同时间点TGFβ1、BMP2的表达水平进行测量比较。结果在不同时间点,颈椎不稳组黄韧带中TGFβ1、BMP2的基因表达较正常黄韧带组均增高(P<0.01)。结论TGFβ1、BMP2在颈椎不稳大鼠黄韧带的表达在不同时间点有明显变化,这种变化可能在加速黄韧带的退变中起重要作用。Objective To study the effects of the unstable cervical spine on transforming growth factor-β1（TGFβ1） and bone morphogenetic protein-2 （BMP2） of the ligamentum flavum in rots. Methods Total RNA was prepared from the ligamentum flavum of the experimental segment of different groups and the expressions of TGFβ1 and BMP2 was determined by reverse transcriptase polymerase chain reaction using specific primers. Result The gene expression of the unstable cervical spine group was higher than that of the control groups at each time spot. Conclusion The expression of TGFβ1 and BMP2 in the ligamentum flavum in rats with an unstable cervical spine is different over time, which may accelerate the degeneration of the ligamentum flavum.

关 键 词：转化生长因子Β1 骨形态发生蛋白2 黄韧带 逆转录聚合酶联反应 

分 类 号：R681.5[医药卫生—骨科学]