TAFI基因编码区C1040T位点单核苷酸多态性与心肌梗死相关性分析  

Association of single nucleotide polymorphism of the C1040T gene encoding TAFI with myocardial infarction

作　　者：王丽丽[1] 徐成伟[1] 于超[1] 赵敬杰[2] 吴晓本[1] 姜翠英[1] 杜贻萌[3] 

机构地区：[1]山东大学第二医院检验科,济南250033 [2]山东大学第二医院临床分子生物学实验室,济南250033 [3]山东大学第二医院心内科,济南250033

出　　处：《山东大学学报（医学版）》2007年第12期1278-1281,共4页Journal of Shandong University:Health Sciences

摘　　要：目的探讨凝血酶激活的纤溶抑制物(TAFI)编码区基因单核苷酸多态性与心肌梗死的相关性。方法应用聚合酶链反应-限制性内切酶片段长度多态性(Restriction fragment length polymorphism,PCR-RFLP)分析技术检测了100例心肌梗死(MI)患者和90例正常对照者的CPB2基因的基因型。结果CPB2基因的3种基因型(Thr325Thr、Thr325Ile、Ile325Ile)频率在MI组和对照组分别为32%、53%、15%和34.4%、54.4%、11.2%,等位基因C、T频率在MI组和对照组分别为58.5%、41.5%和61.7%、38.3%,两组之间基因型和等位基因频率分布差异无统计学意义(P>0.05),且均符合Hardy-Weinberg平衡定律。结论CPB2基因的基因多态性(Thr325Ile)与心肌梗死没有明显关系。Objective To explore the association between encoding gene CPB2 polymorphism of thrombin activatable fibrinolysis inhibitor（TAFI）and myocardial infarction. Methods The PCR-RFLP （Restriction fragment length polymorphism） method was used to determine the CPB2 gene polymorphism in patients with myocardial infarction（ n = 100） and healthy controls（ n = 90）. Results The CPB2 genotype（Thr/Thr,Thr/Ile and Ile/ile）frequencies were 32（32% ）, 53 （53 % ） and 15 （ 15 % ） in the MI group and 31 （ 34.4 % ）, 49 （ 54.4 % ）, 10 （ 11.2 % ） in the control group, and the allelic frequencies（C and T）were 117（58.5%）and 83（41.5%） in the MI group and 111（61.7%）and 69（38.3%）in the control group, x^2 analysis showed non-significant differences in the Thr325Ile polymorphism distributions（ x^2 = 0.648 2, P =0.723 2; g2 = 0. 395 8, P = 0. 529 2） ; genotype and alleles distribution frequencies were in accordance with the Hardy-Weinberg equilibrium. Conclusion Polymorphisms of the TAFI gene are not related to myocardial infarction.

关 键 词：凝血酶激活的纤溶抑制物 基因多态性 心肌梗死 

分 类 号：R541.4[医药卫生—心血管疾病]