芥子碱对鸡胚绒毛尿囊膜血管生成的影响  

Effects of Sinapine on the formation of microvessels in chick embryo chorioallantoic membrane

作　　者：柯木根[1] 陈密玉[1] 吴国欣[1] 林燕妮[1] 

机构地区：[1]福建师范大学生命科学学院,福州350007

出　　处：《中华中医药杂志》2008年第1期36-39,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：福建省自然科学基金资助项目(No.C0410010)

摘　　要：目的:探讨芥子碱对鸡胚绒毛尿囊膜(CAM)新生血管生成的影响。方法:用溶剂萃取法制备芥子碱,通过CAM模型,检测芥子碱对CAM新生血管生成的影响。结果:与生理盐水对照组相比,中、大剂量芥子碱组(5.0、10.0g/L)CAM实验作用部位的血管生成明显受抑,周围血管网模糊,密度减少(P<0.01),抑制率分别达到54.24%和71.62%,而小剂量组(2.5g/L)差异不显著(P>0.05)。结论:芥子碱具有一定的抗血管生成活性,且其作用效果呈剂量依赖性。Objective： To study the influence of Sinapine on the formation of microvessels in chick embryo chorioallantoic membrane （CAM）. Methods： Sinapine was purified by the procedure of pressurized-solvent extraction from Semen Sinapis Albae. The inhibition effect of Sinapine on the chorioaUantoic vessel was detected by the chick embryo chorioallantoic membrane model. Results： Compared with physiological saline group, large areas of blood vessels in Sinapine groups（ 5.0g/L and 10.0g/L） had heavy inhibition of angiogenesis on CAM, accompanied by the decreased density of vessels（ P 〈 0.01 ） with inhibitory rate of 54.24% and 71.62% respectively, but Sinapine group（2.5g/L） didn＇t have the phenomenon（ P 〉 0.05）. Conclusions： Sinapine can prevent the formation of microvessels of CAM and the effects depend on the concentration.

关 键 词：芥子碱 鸡胚绒毛尿囊膜(CAM) 微血管 实验研究 

分 类 号：R285.5[医药卫生—中药学]